Franco Mario Emilio Ernesto, López Silvina Marianela Yanil, Medina Rocio, Lucentini César Gustavo, Troncozo Maria Inés, Pastorino Graciela Noemí, Saparrat Mario Carlos Nazareno, Balatti Pedro Alberto
Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, La Plata, Buenos Aires, Argentina.
Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
PLoS One. 2017 Oct 3;12(10):e0185545. doi: 10.1371/journal.pone.0185545. eCollection 2017.
Stemphylium lycopersici (Pleosporales) is a plant-pathogenic fungus that has been associated with a broad range of plant-hosts worldwide. It is one of the causative agents of gray leaf spot disease in tomato and pepper. The aim of this work was to characterize the mitochondrial genome of S. lycopersici CIDEFI-216, to use it to trace taxonomic relationships with other fungal taxa and to get insights into the evolutionary history of this phytopathogen. The complete mitochondrial genome was assembled into a circular double-stranded DNA molecule of 75,911 bp that harbors a set of 37 protein-coding genes, 2 rRNA genes (rns and rnl) and 28 tRNA genes, which are transcribed from both sense and antisense strands. Remarkably, its gene repertoire lacks both atp8 and atp9, contains a free-standing gene for the ribosomal protein S3 (rps3) and includes 13 genes with homing endonuclease domains that are mostly located within its 15 group I introns. Strikingly, subunits 1 and 2 of cytochrome oxidase are encoded by a single continuous open reading frame (ORF). A comparative mitogenomic analysis revealed the large extent of structural rearrangements among representatives of Pleosporales, showing the plasticity of their mitochondrial genomes. Finally, an exhaustive phylogenetic analysis of the subphylum Pezizomycotina based on mitochondrial data reconstructed their relationships in concordance with several studies based on nuclear data. This is the first report of a mitochondrial genome belonging to a representative of the family Pleosporaceae.
番茄匍柄霉（格孢腔菌目）是一种植物病原真菌，在全球范围内与多种植物宿主相关。它是番茄和辣椒灰叶斑病的病原体之一。这项工作的目的是对番茄匍柄霉CIDEFI - 216的线粒体基因组进行特征分析，利用它来追溯与其他真菌类群的分类关系，并深入了解这种植物病原体的进化历史。完整的线粒体基因组组装成一个75,911 bp的环状双链DNA分子，包含一组37个蛋白质编码基因、2个rRNA基因（rns和rnl）和28个tRNA基因，这些基因从正义链和反义链转录。值得注意的是，其基因库中缺少atp8和atp9，包含一个独立的核糖体蛋白S3（rps3）基因，并且包括13个具有归巢内切酶结构域的基因，这些基因大多位于其15个I组内含子内。引人注目的是，细胞色素氧化酶的亚基1和亚基2由一个连续的开放阅读框（ORF）编码。比较线粒体基因组分析揭示了格孢腔菌目代表之间结构重排的很大程度，显示了它们线粒体基因组的可塑性。最后，基于线粒体数据对盘菌亚门进行的详尽系统发育分析重建了它们的关系，与基于核数据的几项研究结果一致。这是关于格孢腔菌科一个代表的线粒体基因组的首次报道。
