Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
School of Life Sciences, Shanxi University, Taiyuan, Shanxi 030006, China.
Sci Rep. 2017 Jan 10;7:40219. doi: 10.1038/srep40219.
Cordyceps militaris is a fungus used for developing health food, but knowledge about its intraspecific differentiation is limited due to lack of efficient markers. Herein, we assembled the mitochondrial genomes of eight C. militaris strains and performed a comparative mitochondrial genomic analysis together with three previously reported mitochondrial genomes of the fungus. Sizes of the 11 mitochondrial genomes varied from 26.5 to 33.9 kb mainly due to variable intron contents (from two to eight introns per strain). Nucleotide variability varied according to different regions with non-coding regions showing higher variation frequency than coding regions. Recombination events were identified between some locus pairs but seemed not to contribute greatly to genetic variations of the fungus. Based on nucleotide diversity fluctuations across the alignment of all mitochondrial genomes, molecular markers with the potential to be used for future typing studies were determined.
蛹虫草是一种用于开发保健食品的真菌，但由于缺乏有效的标记物，其种内分化的知识有限。在此，我们组装了 8 株蛹虫草的线粒体基因组，并与之前报道的 3 株该真菌的线粒体基因组进行了比较线粒体基因组分析。11 个线粒体基因组的大小从 26.5 到 33.9kb 不等，主要是由于内含子含量的不同（每个菌株有两个到八个内含子）。核苷酸的变异性根据不同的区域而有所不同，非编码区的变异频率高于编码区。在一些基因座对之间鉴定到了重组事件，但似乎对真菌的遗传变异没有很大贡献。根据所有线粒体基因组比对的核苷酸多样性波动，确定了一些具有潜在用于未来分型研究的分子标记。
