Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
J Dent Res. 2012 Jun;91(6):598-604. doi: 10.1177/0022034512444128. Epub 2012 Apr 4.
Antibacterial bonding agents could combat recurrent caries at the tooth-composite margins. The objectives of this study were to develop novel antibacterial dentin primers containing quaternary ammonium dimethacrylate (QADM) and nanoparticles of silver (NAg), and to investigate the effects on dentin bond strength and dental plaque microcosm biofilms for the first time. Scotchbond Multi-Purpose ("SBMP") bonding agent was used. QADM and NAg were incorporated into SBMP primer, yielding 4 primers: SBMP primer (control), control + 10% QADM (mass), control + 0.05% NAg, and control + 10% QADM + 0.05% NAg. Human saliva was collected to grow microcosm biofilms. The NAg particle size (mean ± SD; n = 100) was 2.7 ± 0.6 nm. Dentin shear bond strengths (n = 10) with human third molars were approximately 30 MPa for all groups (p > 0.1). QADM-NAg-containing primer increased the bacteria inhibition zone by 9-fold, compared with control primer (p < 0.05). QADM-NAg-containing primer reduced lactic acid production and colony-forming units of total micro-organisms, total streptococci, and mutans streptococci by an order of magnitude. In conclusion, novel QADM-NAg-containing primers were strongly antibacterial without compromising dentin bond strength, and hence are promising to inhibit biofilms and secondary caries. The processing method of incorporating QADM and NAg together into the same primer produced the strongest antibacterial effect, which could have a wide applicability to other bonding systems.
抗菌粘结剂可防治牙-复合树脂边缘的继发龋。本研究的目的是开发含有季铵基双甲基丙烯酸酯（QADM）和纳米银（NAg）的新型抗菌牙本质底漆，并首次研究其对牙本质粘结强度和牙菌斑微宇宙生物膜的影响。采用 Scotchbond Multi-Purpose（“SBMP”）粘结剂。将 QADM 和 NAg 掺入 SBMP 底漆中，得到 4 种底漆：SBMP 底漆（对照）、对照+10% QADM（质量）、对照+0.05% NAg 和对照+10% QADM+0.05% NAg。收集人唾液以生长微宇宙生物膜。NAg 颗粒大小（平均值±标准差；n = 100）为 2.7 ± 0.6nm。所有组的人第三磨牙牙本质剪切粘结强度（n = 10）均约为 30 MPa（p > 0.1）。与对照底漆相比，含 QADM-NAg 的底漆使细菌抑制区增加了 9 倍（p < 0.05）。含 QADM-NAg 的底漆使总微生物、总链球菌和变形链球菌的乳酸产量和集落形成单位减少了一个数量级。总之，新型含 QADM-NAg 的底漆具有很强的抗菌作用，而不会降低牙本质粘结强度，因此有望抑制生物膜和继发龋。将 QADM 和 NAg 一起共同掺入同一底漆的处理方法产生了最强的抗菌效果，这可能具有广泛的适用性，可以应用于其他粘结系统。
