State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
FEBS Lett. 2013 May 21;587(10):1548-55. doi: 10.1016/j.febslet.2013.01.075. Epub 2013 Apr 12.
Recent studies demonstrated that the mammalian heart possesses some capacity to proliferate. We observed cardiomyocyte proliferation within 4 weeks of age (P4W) in rats. We found 95 microRNAs that are differentially expressed in P4W cardiomyocytes. MicroRNA-29a was among the most highly up-regulated microRNAs in P4W cardiomyocytes. Overexpression of microRNA-29a suppressed the proliferation of H9c2 cell line. MicroRNA-29a inhibition induced cardiomyocytes to proliferate, accelerated the G1/S and G2/M transition, and up-regulated the cell cycle gene expression. Cyclin D2 (CCND2) was identified as a direct target of microRNA-29a. These findings indicate that microRNA-29a is involved in cardiomyocyte proliferation during postnatal development.
最近的研究表明，哺乳动物的心脏具有一定的增殖能力。我们观察到大鼠在出生后 4 周（P4W）时存在心肌细胞增殖。我们发现了 95 种在 P4W 心肌细胞中差异表达的 microRNA。microRNA-29a 是 P4W 心肌细胞中上调最明显的 microRNA 之一。microRNA-29a 的过表达抑制了 H9c2 细胞系的增殖。microRNA-29a 的抑制诱导心肌细胞增殖，加速 G1/S 和 G2/M 转变，并上调细胞周期基因表达。细胞周期蛋白 D2（CCND2）被鉴定为 microRNA-29a 的直接靶标。这些发现表明，microRNA-29a 参与了出生后发育过程中心肌细胞的增殖。
