Wabo Poné J, Payne V K, Mbogning Tayo Gertrude, Komtangi Marie Claire, Yondo Jeannette, Ngangout Alidou M, Mpoame Mbida, Bilong Bilong C F
Laboratory of Applied Biology and Ecology (LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, PO Box 067 Dschang, Cameroon.
Asian Pac J Trop Biomed. 2013 Feb;3(2):100-4. doi: 10.1016/S2221-1691(13)60032-5.
To evaluate the ovicidal and larvicidal activities of aqueous and ethanolic extracts of leaves of Dichrocephala integrifolia (D. integrifolia) against the eggs (fresh and embryonnated), the first and second larval stages of Heligmosomoides bakeri. In order to verify if this medicinal plant possesses active compounds capable of inhibiting the embryonation and hatching of eggs or to induce the mortality of larvae (L1 and L2).
dried extracts were diluted in distilled FIV water to obtain five different concentrations: 625, 1,250, 2,500, 3,750 and 5,000 µg/mL. Fresh eggs obtained from artificially infected mice feces were exposed to these different concentrations for 48 h. Time of contact for embryonated eggs was 6 h while L1 and L2 larvae were exposed for 24 h. Distilled water (placebo) and 1.5% DMSO were used as negative controls.
Distilled water, and 1.5% DMSO had no effect on embryonation, hatching and larval survival. Aqueous extracts of D. integrifolia showed a weak activity against all stages of the parasite at all concentrations tested. On the contrary, the ethanolic extract of D. integrifolia inhibited the embryonation of 87.5% of fresh eggs, the hatching of 81.1% of embryonated eggs and induced the mortality of 98.1% and 98% of L1 and L2 larvae respectively at 5,000 µg/mL.
The results of the present study indicate that the ethanolic extracts of D. integrifolia contained compounds with ovicidal and larvicidal properties. In spite of these results, in vivo tests, studies on toxicity and mechanism of action of active compounds are also needed to validate the utilisation of this medicinal plant by population of Dschang-Cameroon to treat gastro-intestinal parasites.
评估全缘叶马兰（Dichrocephala integrifolia）叶的水提取物和乙醇提取物对巴氏细颈线虫（Heligmosomoides bakeri）的卵（新鲜卵和胚胎卵）、第一和第二幼虫阶段的杀卵和杀幼虫活性。以验证这种药用植物是否拥有能够抑制卵的胚胎发育和孵化或诱导幼虫（L1和L2）死亡的活性化合物。
将干燥提取物用蒸馏水稀释以获得五种不同浓度：625、1250、2500、3750和5000μg/mL。将从人工感染小鼠粪便中获得的新鲜卵暴露于这些不同浓度下48小时。胚胎卵的接触时间为6小时，而L1和L2幼虫暴露24小时。蒸馏水（安慰剂）和1.5%二甲基亚砜用作阴性对照。
蒸馏水和1.5%二甲基亚砜对胚胎发育、孵化和幼虫存活没有影响。在所有测试浓度下，全缘叶马兰的水提取物对寄生虫的所有阶段均表现出较弱的活性。相反，全缘叶马兰的乙醇提取物在5000μg/mL时抑制了87.5%的新鲜卵的胚胎发育、81.1%的胚胎卵的孵化，并分别诱导了98.1%和98%的L1和L2幼虫死亡。
本研究结果表明，全缘叶马兰的乙醇提取物含有具有杀卵和杀幼虫特性的化合物。尽管有这些结果，但仍需要进行体内试验、活性化合物的毒性和作用机制研究，以验证喀麦隆贾格地区人群使用这种药用植物治疗胃肠道寄生虫的合理性。
