Department of Chemistry, Dyal Singh College, University of Delhi, Lodhi Road, Delhi, 110003, India.
J Biomater Sci Polym Ed. 2013;24(7):865-81. doi: 10.1080/09205063.2012.723958. Epub 2012 Sep 12.
In this article, a sustained release formulation of the antioxidant gallic acid (GA) is presented in the form of glutathione responsive disulfide cross-linked poly(ethylene glycol)-based nanogels synthesized via aqueous inverse miniemulsion using atom transfer radical polymerization. The particle size was found to be in the range from 227 ± 51.78 to 573.3 ± 207.2 nm at three drug loading levels achieved i.e. 6.6, 14.26, and 18.29 wt.% of the nanogels with loading efficiency in the range of 60-70%. The release profile of the GA studied at three drug loading levels suggested a controlled release and the nanogels were capable of scavenging radicals and retained the antioxidant activity. The GA-loaded nanogels were found to be biocompatible on human cervical cancer cell lines (HeLa). DCFH-DA (2,7-dichlorofluorescin diacetate) assay evidenced that the nanogels were capable of scavenging the reactive oxygen species in cellular environment.
本文以谷胱甘肽响应的二硫键交联聚乙二醇基纳米凝胶的形式呈现了抗氧化剂没食子酸（GA）的缓释制剂，该纳米凝胶是通过原子转移自由基聚合在水包油反相微乳液中合成的。在三种药物载药量（即纳米凝胶的 6.6、14.26 和 18.29wt.%）下，发现粒径在 227±51.78 至 573.3±207.2nm 范围内，载药效率在 60-70%之间。在三种药物载药量下研究的 GA 释放曲线表明其具有控制释放特性，且纳米凝胶能够清除自由基并保持抗氧化活性。负载 GA 的纳米凝胶在人宫颈癌细胞系（HeLa）上表现出良好的生物相容性。DCFH-DA（2,7-二氯荧光素二乙酸酯）实验证明，纳米凝胶能够在细胞环境中清除活性氧。
