Valencia-Llano Carlos Humberto, Solano Moisés A, Grande-Tovar Carlos David
Grupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 76001, Colombia.
Grupo de Investigación de Fotoquímica y Fotobiología, Facultad de Ciencias, Programa de Química, Universidad del Atlántico, Carrera 30 Número 8-49, Puerto Colombia 081008, Colombia.
Polymers (Basel). 2021 Nov 10;13(22):3877. doi: 10.3390/polym13223877.
New technologies based on nanocomposites of biopolymers and nanoparticles inspired by the nature of bone structure have accelerated their application in regenerative medicine, thanks to the introduction of reinforcing properties. Our research incorporated chitosan (CS) covalently crosslinked with glutaraldehyde (GLA) beads with graphene oxide (GO) nanosheets, titanium dioxide nanoparticles (TiO), and blackberry processing waste extract (BBE) and evaluated them as partial bone substitutes. Skullbone defects in biomodels filled with the scaffolds showed evidence through light microscopy, scanning electron microscopy, histological studies, soft tissue development with hair recovery, and absence of necrotic areas or aggressive infectious response of the immune system after 90 days of implantation. More interestingly, newly formed bone was evidenced by elemental analysis and Masson trichromacy analysis, which demonstrated a possible osteoinductive effect from the beads using the critical size defect experimental design in the biomodels. The results of this research are auspicious for the development of bone substitutes and evidence that the technologies for tissue regeneration, including chitosan nanocomposites, are beneficial for the adhesion and proliferation of bone cells.
受骨结构性质启发的基于生物聚合物和纳米颗粒的纳米复合材料的新技术，由于增强性能的引入，加速了它们在再生医学中的应用。我们的研究将与戊二醛（GLA）珠共价交联的壳聚糖（CS）与氧化石墨烯（GO）纳米片、二氧化钛纳米颗粒（TiO）和黑莓加工废料提取物（BBE）相结合，并将它们评估为部分骨替代物。植入90天后，填充有支架的生物模型中的颅骨缺损通过光学显微镜、扫描电子显微镜、组织学研究、毛发恢复的软组织发育以及免疫系统无坏死区域或侵袭性感染反应得到证实。更有趣的是，通过元素分析和马松三色染色分析证明了新形成的骨，这表明在生物模型中使用临界尺寸缺损实验设计，珠子可能具有骨诱导作用。这项研究的结果对于骨替代物的开发是吉祥的，并且证明包括壳聚糖纳米复合材料在内的组织再生技术有利于骨细胞的粘附和增殖。
