Murthy Hosakatte Niranjana, Joseph Kadanthottu Sebastian, Paek Kee Yoeup, Park So Young
Department of Botany, Karnatak University, Dharwad, India.
Department of Horticultural Science, Chungbuk National University, Cheongju, Republic of Korea.
Crit Rev Biotechnol. 2024 Aug;44(5):837-859. doi: 10.1080/07388551.2023.2233690. Epub 2023 Jul 27.
plant cell and organ cultures are appealing alternatives to traditional methods of producing valuable specialized metabolites for use as: pharmaceuticals, food additives, cosmetics, perfumes, and agricultural chemicals. Cell cultures have been adopted for the production of specialized metabolites in certain plants. However, in certain other systems, adventitious roots are superior to cell suspension cultures as they are organized structures that accumulate high levels of specialized metabolites. The cultivation of adventitious roots has been investigated in various bioreactor systems, including: mechanically agitated, pneumatically agitated, and modified bioreactors. The main relevance and importance of this work are to develop a long-lasting industrial biotechnological technology as well as to improve the synthesis of these metabolites from the plant systems. These challenges are exacerbated by: the peculiarities of plant cell metabolism, the complexity of specialized metabolite pathways, the proper selection of bioreactor systems, and bioprocess optimization. This review's major objective is to analyze several bioreactor types for the development of adventitious roots, as well as the advantages and disadvantages of each type of bioreactor, and to describe the strategies used to increase the synthesis of specialized metabolites. This review also emphasizes current advancements in the field, and successful instances of scaled-up cultures and the generation of specialized metabolites for commercial purposes are also covered.
植物细胞和器官培养是生产有价值的特殊代谢产物的传统方法的有吸引力的替代方法，这些特殊代谢产物可用于：制药、食品添加剂、化妆品、香水和农用化学品。细胞培养已被用于某些植物中特殊代谢产物的生产。然而，在某些其他系统中，不定根优于细胞悬浮培养，因为它们是积累高水平特殊代谢产物的组织结构。不定根的培养已在各种生物反应器系统中进行了研究，包括：机械搅拌、气动搅拌和改良生物反应器。这项工作的主要相关性和重要性在于开发一种持久的工业生物技术，以及改善这些代谢产物在植物系统中的合成。植物细胞代谢的特殊性、特殊代谢产物途径的复杂性、生物反应器系统的正确选择和生物过程优化加剧了这些挑战。本综述的主要目的是分析几种用于不定根培养的生物反应器类型，以及每种类型生物反应器的优缺点，并描述用于增加特殊代谢产物合成的策略。本综述还强调了该领域的当前进展，并且还涵盖了扩大培养规模和生产用于商业目的的特殊代谢产物的成功实例。
