摇瓶培养中 3-乙酰脱氧雪腐镰刀菌烯醇的生成。
Production of 3-acetyldeoxynivalenol in shake culture.
机构信息
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Shinjuku-ku, 162, Tokyo, Japan.
出版信息
Mycotoxin Res. 1985 Mar;1(1):19-24. doi: 10.1007/BF03191950.
In order to produce deoxynivalenol (DON), conditions for the production of 3-acetyldeoxynivalenol (Ac-DON) in shake culture were studied. A selected isolate of DON-producer,F. graminearum strain R2118, produced about 70mg/mL of Ac-DON when cultured at 25°C for 4 days in a medium consisting of 3% sucrose, 0.1% peptone, and 0.1% yeast extract. From the mass culture (10 L) of the strain, 792 mg of Ac-DON were isolated and 384 mg of DON were obtained after hydrolysis of the acetate.
为了生产脱氧雪腐镰刀菌烯醇（DON），研究了在摇瓶培养中产生 3-乙酰脱氧雪腐镰刀菌烯醇（Ac-DON）的条件。一株 DON 产生菌的选择性分离株，禾谷镰刀菌菌株 R2118，在 25°C 的培养基中培养 4 天，该培养基由 3%蔗糖、0.1%蛋白胨和 0.1%酵母提取物组成，可产生约 70mg/mL 的 Ac-DON。从该菌株的大规模培养（10 L）中，分离得到 792 mg 的 Ac-DON，经水解乙酸酯后得到 384 mg 的 DON。
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