Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China.
Anim Sci J. 2013 Jul;84(7):535-42. doi: 10.1111/asj.12043. Epub 2013 Mar 18.
Krüppel-like factor 7 (KLF7) has been extensively studied in mammalian species, but its role in birds is still unclear. In the current study, cloning and sequencing showed that the full-length coding region of chicken KLF7 (Gallus gallus KLF7, gKLF7) was 891 bp long, encoding 296 amino acids. In addition, real-time RT-PCR analysis showed that gKLF7 was broadly expressed in all 15 chicken tissues selected, and its expression was significantly different in spleen, proventriculus, abdominal fat, brain, leg muscle, gizzard and heart between fat and lean broilers at 7 weeks of age. Additionally, one novel single nucleotide polymorphism (SNP), XM_426569.3: c. A141G, was identified in the second exon of gKLF7. Association analysis showed that this locus was significantly associated with fatness traits in Arbor Acres broiler random population and the eighth generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population (P < 0.05). These results suggest that gKLF7 might be a candidate gene for chicken fatness traits.
Krüppel 样因子 7（KLF7）在哺乳动物物种中得到了广泛研究，但它在鸟类中的作用尚不清楚。在本研究中，通过克隆和测序表明，鸡 KLF7（Gallus gallus KLF7，gKLF7）的全长编码区长 891bp，编码 296 个氨基酸。此外，实时 RT-PCR 分析表明，gKLF7 在所选的 15 种鸡组织中广泛表达，在 7 周龄的肥、瘦肉鸡之间，其在脾脏、腺胃、腹脂、大脑、腿肌、肌胃和心脏中的表达存在显著差异。此外，在 gKLF7 的第二外显子中发现了一个新的单核苷酸多态性（SNP），XM_426569.3：c. A141G。关联分析表明，该位点与阿伯丁安格斯肉鸡随机群体和东北农业大学肉鸡系第八代腹部脂肪含量（NEAUHLF）群体的肥胖性状显著相关（P<0.05）。这些结果表明，gKLF7 可能是鸡肥胖性状的候选基因。
