Leng L, Wang S, Li Z, Wang Q, Li H
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P. R. China.
Poult Sci. 2009 May;88(5):938-42. doi: 10.3382/ps.2008-00453.
Insulin-like growth factor binding protein 2 (IGFBP2) is the predominant insulin-like growth factor binding protein in serum of different species. It binds insulin-like growth factors and plays an important role in growth and fat metabolism. The current study was designed to investigate the associations of IGFBP2 gene 3'-flanking region polymorphisms with chicken fatness traits. The random populations (5 distinct populations: Arbor Acres broiler, Hyline Brown layer, Shiqiza, BeijingYou, and Baier), the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF), and the the Northeast Agricultural University F(2) (NEAU F(2)) resource population were used in the current study. Body weight and body composition traits were measured in NEAUHLF and NEAU F(2) populations. A SNP of 1196C > A in the 3'-flanking region of the IGFBP2 gene was detected, and the PCR-single strand conformation polymorphism method was then used to genotype all of the individuals derived from the above populations. There was a highly significant difference (P < 0.01) in the allele frequency among the 5 distinct populations. The IG-FBP2 polymorphism was significantly associated with abdominal fat weight and percentage of abdominal fat (P < 0.05) in NEAUHLF and NEAU F(2) populations. The research suggests that the IGFBP2 gene could be a candidate locus or linked to a major gene that affects fatness traits in chickens.
胰岛素样生长因子结合蛋白2（IGFBP2）是不同物种血清中主要的胰岛素样生长因子结合蛋白。它能结合胰岛素样生长因子，在生长和脂肪代谢中发挥重要作用。本研究旨在探讨IGFBP2基因3'侧翼区多态性与鸡脂肪性状的关联。本研究使用了随机群体（5个不同群体：艾维茵肉鸡、海兰褐蛋鸡、石岐杂鸡、北京油鸡和白耳鸡）、东北农业大学对腹脂含量进行双向选择的肉鸡品系（NEAUHLF）以及东北农业大学F2（NEAU F2）资源群体。在NEAUHLF和NEAU F2群体中测量了体重和体组成性状。检测到IGFBP2基因3'侧翼区1196C>A的单核苷酸多态性（SNP），然后使用聚合酶链反应-单链构象多态性方法对上述群体的所有个体进行基因分型。5个不同群体的等位基因频率存在极显著差异（P<0.01）。在NEAUHLF和NEAU F2群体中，IGFBP2多态性与腹脂重量和腹脂百分比显著相关（P<0.05）。该研究表明，IGFBP2基因可能是影响鸡脂肪性状的一个候选基因座或与一个主基因连锁。
