Liang Shu-Hui, Yan Xi-Zhang, Wang Biao-Luo, Jin Hai-Feng, Yao Li-Ping, Li Ya-Ni, Chen Min, Nie Yong-Zhan, Wang Xin, Guo Xue-Gang, Wu Kai-Chun, Ding Jie, Fan Dai-Ming
State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, Fourth Military Medical University, No. 127 Changle Western Rd., Xi'an, 710032, Shaanxi Province, China.
Tumour Biol. 2013 Oct;34(5):2605-9. doi: 10.1007/s13277-013-0808-x. Epub 2013 Apr 23.
Altered expression of forkhead box Q1 (FOXQ1) is observed in various types of human cancers. However, the clinical significance of FOXQ1 expression in gastric cancer (GC) remains largely unknown. The present study aims to explore the clinicopathological significance and prognostic value of FOXQ1 in GC. FOXQ1 messenger RNA (mRNA) and protein expression were determined by quantitative real-time reverse transcriptase-polymerase chain reaction and Western blot in 20 pairs of fresh frozen GC tissues and corresponding noncancerous tissues. Additionally, FOXQ1 expression was analyzed by immunohistochemistry in 158 clinicopathologically characterized GC cases. The correlation of FOXQ1 expression with patients' survival rate was assessed by Kaplan-Meier and Cox regression. Our results showed that the expression levels of FOXQ1 mRNA and protein in GC tissues were both significantly higher than those in non-cancerous tissues. Our results showed that the high expression of FOXQ1 in GC was related to tumor size (P = 0.026), histological grade (P = 0.021), lymph node involvement (P = 0.002), and tumor-node-metastasis stage (P = 0.028). Kaplan-Meier survival analysis showed that a high expression level of FOXQ1 resulted in a significantly poor prognosis of GC patients. Furthermore, Cox multivariates analysis indicated that FOXQ1 expression level was an independent prognostic factor for the overall survival rate of GC patients. In conclusion, overexpression of FOXQ1 is closely related to progression of GC and might be regarded as an independent predictor of poor prognosis for GC.
在多种人类癌症中均观察到叉头框Q1（FOXQ1）表达改变。然而，FOXQ1在胃癌（GC）中表达的临床意义仍 largely未知。本研究旨在探讨FOXQ1在GC中的临床病理意义及预后价值。通过定量实时逆转录聚合酶链反应和蛋白质印迹法测定了20对新鲜冷冻的GC组织及相应癌旁组织中FOXQ1信使核糖核酸（mRNA）和蛋白质的表达。此外，通过免疫组织化学分析了158例具有临床病理特征的GC病例中FOXQ1的表达。采用Kaplan-Meier法和Cox回归评估FOXQ1表达与患者生存率的相关性。我们的结果显示，GC组织中FOXQ1 mRNA和蛋白质的表达水平均显著高于癌旁组织。我们的结果显示，GC中FOXQ1的高表达与肿瘤大小（P = 0.026）、组织学分级（P = 0.021）、淋巴结受累（P = 0.002）及肿瘤-淋巴结-转移分期（P = 0.028）相关。Kaplan-Meier生存分析显示，FOXQ1高表达导致GC患者预后显著较差。此外，Cox多因素分析表明，FOXQ1表达水平是GC患者总生存率的独立预后因素。总之，FOXQ1过表达与GC进展密切相关，可能被视为GC预后不良的独立预测指标。
