Exp Dermatol. 2013 May;22(5):374-6. doi: 10.1111/exd.12139.
The transcription factor p63 plays an essential role in maintaining the proliferative potential of epidermal stem cells. We have shown recently that under homoeostatic conditions, phosphorylation of p63 increases during the early transition of stem cells to transit-amplifying cells in human epidermis. However, how p63 phosphorylation relates to the regenerative processes during wound healing remains unknown. In this study, we characterize epidermal cells that contribute to wound repair in mouse models using phosphorylated p63 as a marker for stem cell differentiation. Our studies reveal that epidermal progenitors with high p63 phosphorylation preferentially expand in response to wounding in both full-thickness wound and surface injury models. As phosphorylated p63 levels inversely correlate with the proliferative potential of epidermal progenitors, p63 phosphorylation may serve as a therapeutic target to modulate the function of these regenerative cells during wound healing.
转录因子 p63 在维持表皮干细胞的增殖潜能方面发挥着重要作用。我们最近表明，在同型平衡条件下，p63 的磷酸化在人表皮中干细胞向过渡扩增细胞的早期转变过程中增加。然而，p63 磷酸化与伤口愈合过程中的再生过程如何相关尚不清楚。在这项研究中，我们使用磷酸化 p63 作为干细胞分化的标志物，来描绘参与小鼠模型伤口修复的表皮细胞。我们的研究表明，在全层伤口和表面损伤模型中，高磷酸化 p63 的表皮祖细胞优先在受到创伤后扩增。由于磷酸化 p63 水平与表皮祖细胞的增殖潜能呈反比，p63 磷酸化可能成为一种治疗靶点，以调节这些再生细胞在伤口愈合过程中的功能。
