Department of Preventive Medicine and Public Health, School of Pharmacy, University of Granada, Granada, Spain. elmorol@.ugr.es
Foodborne Pathog Dis. 2013 Jun;10(6):528-32. doi: 10.1089/fpd.2012.1376. Epub 2013 Apr 24.
Viruses are the leading cause of foodborne illness associated with the consumption of raw or slightly cooked contaminated shellfish. The aim of this study was to evaluate the prevalence of hepatitis A virus in molluscs. Standard and real-time reverse transcription-polymerase chain reaction procedures were used to monitor bivalve molluscs from the Granada fish markets (southern Spain) for this human enteric virus. Between February 2009 and October 2010, we collected a total of 329 samples of different types of bivalve molluscs (mussels, smooth clams, striped venus, and grooved clams). The results showed the presence of hepatitis A virus in 8.5% of the 329 samples analyzed. We can therefore confirm that conventional fecal indicators are unreliable for demonstrating the presence or absence of viruses. The presence of hepatitis A virus in molluscs destined for human consumption is a potential health risk in southern Spain.
病毒是导致与食用生的或轻微煮熟的受污染贝类相关的食源性疾病的主要原因。本研究旨在评估甲型肝炎病毒在贝类中的流行情况。采用标准和实时逆转录-聚合酶链反应程序监测来自西班牙南部格拉纳达鱼市的双壳贝类中的这种人类肠道病毒。在 2009 年 2 月至 2010 年 10 月期间，我们共收集了 329 份不同类型的双壳贝类（贻贝、光滑蛤、条纹维纳斯蛤和沟纹蛤）的样本。结果显示，在分析的 329 份样本中，有 8.5%的样本存在甲型肝炎病毒。因此，我们可以确认传统的粪便指标不可靠，无法证明病毒的存在或不存在。甲型肝炎病毒在供人类食用的贝类中的存在是西班牙南部的一个潜在健康风险。
