Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, 412007, People's Republic of China.
Nanoscale Res Lett. 2013 Apr 24;8(1):190. doi: 10.1186/1556-276X-8-190.
The purpose of this study is to determine if amino acid neurotransmitters such as gamma-aminobutyric acid (GABA), taurine, glutamate (Glu), and aspartate (Asp) can scavenge activated carbonyl toxicants. In vitro, direct reaction between malondialdehyde (MDA) and amino acids was researched using different analytical methods. The results indicated that scavenging activated carbonyl function of taurine and GABA is very strong and that of Glu and Asp is very weak in pathophysiological situations. The results provided perspective into the reaction mechanism of taurine and GABA as targets of activated carbonyl such as MDA in protecting nerve terminals. In vivo, we studied the effect of taurine and GABA as antioxidants by detecting MDA concentration and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. It was shown that MDA concentration was decreased significantly, and the activities of SOD and GSH-Px were increased significantly in the cerebral cortex and hippocampus of acute epileptic state rats, after the administration of taurine and GABA. The results indicated that the peripherally administered taurine and GABA can scavenge free radicals and protect the tissue against activated carbonyl in vivo and in vitro.
本研究旨在确定氨基酸神经递质如γ-氨基丁酸（GABA）、牛磺酸、谷氨酸（Glu）和天冬氨酸（Asp）是否可以清除活性羰基毒素。在体外，使用不同的分析方法研究了丙二醛（MDA）与氨基酸之间的直接反应。结果表明，在病理生理情况下，牛磺酸和 GABA 清除活性羰基功能非常强，而 Glu 和 Asp 则非常弱。这些结果为牛磺酸和 GABA 作为 MDA 等活性羰基的靶标在保护神经末梢方面的反应机制提供了新的视角。在体内，我们通过检测丙二醛（MDA）浓度和超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性来研究牛磺酸和 GABA 作为抗氧化剂的作用。结果表明，在急性癫痫状态大鼠的大脑皮质和海马中，给予牛磺酸和 GABA 后，MDA 浓度显著降低，SOD 和 GSH-Px 的活性显著升高。这些结果表明，外周给予的牛磺酸和 GABA 可以在体内和体外清除自由基，并保护组织免受活性羰基的侵害。
