Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Anal Chim Acta. 2013 May 13;777:1-16. doi: 10.1016/j.aca.2013.01.025. Epub 2013 Jan 23.
Increasing interest has been paid to label-free biosensors in recent years. Among them, refractive index (RI) optical biosensors enable high density and the chip-scale integration of optical components. This makes them more appealing to help develop lab-on-a-chip devices. Today, many RI integrated optical (IO) devices are made using silicon-based materials. A key issue in their development is the biofunctionalization of sensing surfaces because they provide a specific, sensitive response to the analyte of interest. This review critically discusses the biofunctionalization procedures, assay formats and characterization techniques employed in setting up IO biosensors. In addition, it provides the most relevant results obtained from using these devices for real sample biosensing. Finally, an overview of the most promising future developments in the fields of chemical surface modification and capture agent attachment for IO biosensors follows.
近年来，人们对无标记生物传感器越来越感兴趣。其中，折射率（RI）光学生物传感器能够实现高密度和芯片级的光学元件集成。这使得它们更具吸引力，有助于开发片上实验室设备。如今，许多 RI 集成光学（IO）设备都是使用硅基材料制造的。它们发展的一个关键问题是传感表面的生物功能化，因为它们对感兴趣的分析物提供了特定的、敏感的响应。本文批判性地讨论了用于建立 IO 生物传感器的生物功能化程序、分析方法和表征技术。此外，它还提供了使用这些设备进行实际样品生物传感的最相关结果。最后，概述了 IO 生物传感器化学表面修饰和捕获剂附着领域最有前途的未来发展。
