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使用回音壁模式生物传感器进行定量生物标志物检测开发指南。

A Guide to Quantitative Biomarker Assay Development using Whispering Gallery Mode Biosensors.

作者信息

Robison Heather M, Bailey Ryan C

机构信息

Department of Chemistry, University of Illinois at Urbana-Champaign.

Department of Chemistry, University of Michigan, Ann Arbor.

出版信息

Curr Protoc Chem Biol. 2017 Sep 14;9(3):158-173. doi: 10.1002/cpch.23.

Abstract

Whispering gallery mode (WGM) sensors are a class of powerful analytical techniques defined by the measurement of changes in the local refractive index at or near the sensor surface. When functionalized with target-specific capture agents, analyte binding can be measured with very low limits of detection. There are many geometric manifestations of WGM sensors, with chip-integrated silicon photonic devices first commercialized because of the robust, wafer-scale device fabrication, facile optical interrogation, and amenability to the creation of multiplexed sensor arrays. Using these arrays, a number of biomolecular targets have been detected in both label-free and label-enhanced assay formats. For example, sub-picomolar detection limits for multiple cytokines were achieved using an enzymatically enhanced sandwich immunoassay that showed high analyte specificity suitable for detection in complex, clinical matrices. This protocol describes a generalizable approach for the development of quantitative, multiplexed immunoassays using silicon photonic microrings as an example WGM platform. © 2017 by John Wiley & Sons, Inc.

摘要

回音壁模式(WGM)传感器是一类强大的分析技术,其定义为测量传感器表面或附近局部折射率的变化。当用目标特异性捕获剂进行功能化时,可以以非常低的检测限测量分析物结合。WGM传感器有许多几何表现形式,芯片集成硅光子器件首先实现商业化,这是因为其具有强大的晶圆级器件制造能力、便捷的光学检测以及易于创建多路复用传感器阵列。使用这些阵列,已经在无标记和标记增强检测形式中检测到了许多生物分子靶标。例如,使用酶促增强夹心免疫测定法实现了多种细胞因子的亚皮摩尔检测限,该方法显示出适用于在复杂临床基质中检测的高分析物特异性。本方案描述了一种以硅光子微环作为示例WGM平台开发定量、多路复用免疫测定法的通用方法。© 2017约翰威立国际出版公司

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