Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino, Italy.
Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2723-35. doi: 10.1016/j.msec.2013.02.039. Epub 2013 Feb 28.
Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327±45 nm and 1.64±0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).
明胶（GL）纳米纤维基质通过静电纺丝技术从水溶液中制备，模仿天然细胞外基质（ECM）的复杂生物结构。通过优化工艺和溶液参数，获得直径约 300nm 的 GL 纳米纤维。为了提高 GL 在水环境中的稳定性，γ-缩水甘油醚氧丙基三甲氧基硅烷（GPTMS）被用作 GL 交联剂。GPTMS 交联并未改变纳米纤维基质的形态：纤维直径和膜孔尺寸分别为 327±45nm 和 1.64±0.37μm。所制备的 GPTMS 交联 GL 纳米纤维（GL/GPTMS_NF）被发现支持体外新生嗅球包被细胞（NOBECs）的黏附、增殖和存活。
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