Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2995-3000. doi: 10.1016/j.msec.2013.03.026. Epub 2013 Mar 23.
Bacterial nanocellulose (BNC) was biosynthesized by Gluconacetobacter xylinus. The surface area, physicochemical structure and morphology of the materials were characterized. Here provides a method for an efficient production of uniform BNC, which is beneficial for the fast characterization and evaluation of BNC. In vitro cytotoxicity of the materials was evaluated by the proliferation, the adhesion, the viability and the morphology of NIH/3T3 cells. Low cytotoxicity of the BNC was observed, and micrographs demonstrate a good proliferation and adhesion of NIH/3T3 cells on BNC. Large area full-thickness skin defects were made on the back of C57BL/6 mice in animal surgery. The wounds were transplanted with BNC films and the results compared to those in a control group. The rehabilitation of the wound surfaces and the pathological sections of mice were investigated and are discussed. Histological examinations demonstrated faster and better healing effect and lower inflammatory response in the BNC group than those in the control group. Preliminary results on wound dressings from BNC show a curative effect promoting the healing of epithelial tissue. BNC is a promising natural polymer with medical applications in wound dressings.
细菌纳米纤维素 (BNC) 由木醋酸杆菌生物合成。对材料的比表面积、理化结构和形貌进行了表征。本研究提供了一种高效生产均匀 BNC 的方法，有利于 BNC 的快速表征和评价。通过 NIH/3T3 细胞的增殖、黏附、活力和形态评估了材料的体外细胞毒性。结果表明 BNC 具有低细胞毒性，显微镜照片显示 NIH/3T3 细胞在 BNC 上具有良好的增殖和黏附能力。在动物手术中，在 C57BL/6 小鼠背部制造大面积全层皮肤缺损。将 BNC 薄膜移植到伤口上，并与对照组进行比较。研究并讨论了伤口表面和小鼠病理切片的恢复情况。组织学检查表明，BNC 组的愈合效果更快更好，炎症反应更低。初步研究结果表明，BNC 在伤口敷料方面具有促进上皮组织愈合的疗效。BNC 是一种有前途的天然聚合物，在伤口敷料中有应用前景。
