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采用生物信息学方法研究生物合成的 BNC/FeO 纳米复合材料辅助下的伤口愈合的分子研究。

Molecular study of wound healing after using biosynthesized BNC/FeO nanocomposites assisted with a bioinformatics approach.

机构信息

Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Young Researcher and Elite Club, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

出版信息

Int J Nanomedicine. 2018 May 21;13:2955-2971. doi: 10.2147/IJN.S159637. eCollection 2018.

DOI:10.2147/IJN.S159637
PMID:29861630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5968787/
Abstract

BACKGROUND

Molecular investigation of wound healing has allowed better understanding about interaction of genes and pathways involved in healing progression.

OBJECTIVES

The aim of this study was to prepare magnetic/bacterial nanocellulose (FeO/BNC) nanocomposite films as ecofriendly wound dressing in order to evaluate their physical, cytotoxicity and antimicrobial properties. The molecular study was carried out to evaluate expression of genes involved in healing of wounds after treatment with BNC/FeO films.

STUDY DESIGN MATERIALS AND METHODS

Magnetic nanoparticles were biosynthesized by using extract in new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Moreover, swelling property and metal ions release profile of the nanocomposites were investigated. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was examined. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR.

RESULTS

Spherical magnetic nanoparticles (15-30 nm) were formed and immobilized within the structure of BNC. The BNC/FeO was nontoxic (IC>500 μg/mL) with excellent wound healing efficiency after 48 hours. The nanocomposites showed good antibacterial activity ranging from 6±0.2 to 13.40±0.10 mm against , and . The effective genes for the wound healing process were , , , , , , and with time dependent manner. BNC/FeO has an effect on microRNA by reducing its expression and therefore causing an increase in the gene expression of other genes, which consequently resulted in wound healing.

CONCLUSION

This eco-friendly nanocomposite with excellent healing properties can be used as an effective wound dressing for treatment of cutaneous wounds.

摘要

背景

分子研究对伤口愈合的作用，使人们更好地了解参与愈合进程的基因和途径之间的相互作用。

目的

本研究旨在制备磁性/细菌纳米纤维素（FeO/BNC）纳米复合材料薄膜作为环保型伤口敷料，以评估其物理性能、细胞毒性和抗菌性能。通过分子研究，评估了治疗后 BNC/FeO 薄膜对伤口愈合相关基因表达的影响。

研究设计

采用新分离的细菌纳米纤维素（BNC）RM1 的 提取物生物合成磁性纳米颗粒。采用 X 射线衍射、傅里叶变换红外和场发射扫描电子显微镜对纳米复合材料进行了表征。此外，还研究了纳米复合材料的溶胀性能和金属离子释放特性。通过体外人真皮成纤维细胞实验，考察了纳米复合材料促进伤口愈合的能力。利用生物信息学数据库鉴定了具有重要愈合效果的基因。通过定量实时 PCR 研究了干扰愈合的关键基因。

结果

形成了 15-30nm 的球形磁性纳米颗粒，并固定在 BNC 的结构内。BCN/FeO 在 48 小时后对细胞无毒性（IC>500μg/mL），具有良好的伤口愈合效率。纳米复合材料对 、 和 表现出良好的抗菌活性，抑菌圈直径分别为 6±0.2 至 13.40±0.10mm。 、 、 、 、 和 等有效基因与时间呈正相关。BCN/FeO 通过降低其表达从而增加其他基因的表达，对微 RNA 有一定的影响，进而导致伤口愈合。

结论

这种具有优异愈合性能的环保型纳米复合材料可用作治疗皮肤伤口的有效伤口敷料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/5f24638bedda/ijn-13-2955Fig14.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/5f24638bedda/ijn-13-2955Fig14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/f960dfb72a3b/ijn-13-2955Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/5beeab186e72/ijn-13-2955Fig2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/e976ee5354e6/ijn-13-2955Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/6bd2f825591a/ijn-13-2955Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/865565698039/ijn-13-2955Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/275b16888aeb/ijn-13-2955Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/24cfc5ad9eb7/ijn-13-2955Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/93b9de6490ae/ijn-13-2955Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/95b6637b0f79/ijn-13-2955Fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/2421cc502579/ijn-13-2955Fig12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/1795a12e5ea2/ijn-13-2955Fig13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba93/5968787/5f24638bedda/ijn-13-2955Fig14.jpg

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