Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Small. 2013 Sep 23;9(18):3093-102. doi: 10.1002/smll.201300171. Epub 2013 Apr 26.
A highly emissive far-red/near-infrared (FR/NIR) fluorescent conjugated polymer (CP), poly[(9,9-dihexylfluorene)-co-2,1,3-benzothiadiazole-co-4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole] (PFBTDBT10) is designed and synthesized via Suzuki polymerization. Formulation of PFBTDBT10 using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000 ) and DSPE-PEG5000-folate as the encapsulation matrix yielded CP-loaded DSPE-PEG-folic acid nanoparticles (CPDP-FA NPs) with bright FR/NIR fluorescence (27% quantum yield) and a large Stoke's shift of 233 nm in aqueous solution. CPDP-FA NPs show improved thermal/photostabilities and larger Stoke's shifts as compared to commercially available quantum dots (Qdot 655) and organic dyes such as Alexa Fluor 555 and Rhodamine 6G. In vivo studies of CPDP-FA NPs on a hepatoma H22 tumor-bearing mouse model reveal that they could serve as an efficient FR/NIR fluorescent probe for targeted in vivo fluorescence imaging and cancer detection in a high contrast and specific manner. Together with the negligible in vivo toxicity, CPDP-FA NPs are promising FR/NIR fluorescent probes for future in vivo applications.
一种具有高发光效率的远红/近红外（FR/NIR）荧光共轭聚合物（CP），聚[（9,9-二己基芴）-共-2,1,3-苯并噻二唑-共-4,7-二（噻吩-2-基）-2,1,3-苯并噻二唑]（PFBTDBT10），通过 Suzuki 聚合反应设计并合成。通过使用 1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-[甲氧基（聚乙二醇）-2000]（DSPE-PEG2000）和 DSPE-PEG5000-叶酸作为封装基质，将 PFBTDBT10 制剂化为具有明亮的 FR/NIR 荧光（27%量子产率）和在水溶液中具有 233nm 大斯托克斯位移的 CP 负载的 DSPE-PEG-叶酸纳米颗粒（CPDP-FA NPs）。与市售量子点（Qdot 655）和有机染料如 Alexa Fluor 555 和 Rhodamine 6G 相比，CPDP-FA NPs 具有改善的热/光稳定性和更大的斯托克斯位移。在荷肝癌 H22 肿瘤小鼠模型上进行的 CPDP-FA NPs 的体内研究表明，它们可用作高效的 FR/NIR 荧光探针，以靶向进行体内荧光成像和癌症检测，具有高对比度和特异性。CPDP-FA NPs 具有可忽略的体内毒性，是未来体内应用的有前途的 FR/NIR 荧光探针。
