Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143-701, Republic of Korea.
Analyst. 2013 Jun 21;138(12):3347-55. doi: 10.1039/c3an00077j. Epub 2013 Apr 29.
Accelerator mass spectrometry (AMS) is the ultimate technique for measuring rare isotopes in small samples. Biological and biomedical applications of (14)C-AMS (bio-(14)C-AMS) commenced in the early 1990s and are now widely used in many research fields including pharmacology, toxicology, food, and nutrition. For accurate, precise, and reproducible bio-(14)C-AMS analysis, the graphitization step in sample preparation is the most critical step. So, various sample preparation methods for a process called graphitization have been reported for specific applications. Catalytic graphitization using either a flame-sealed borosilicate tube or a septa-sealed vial is a popular sample preparation method for bio-(14)C-AMS. In this review, we introduce the AMS system, especially for bio-(14)C-AMS. In addition, we also review the graphitization method for bio-(14)C-AMS to promote further understanding and improvement of sample preparation for this technique. Examples of catalytic graphitization methods over the past two decades are described.
加速器质谱 (AMS) 是测量小样本中稀有同位素的终极技术。（14）C-AMS（生物-（14）C-AMS）的生物和生物医学应用始于 20 世纪 90 年代初，现在广泛应用于药理学、毒理学、食品和营养等许多研究领域。为了进行准确、精确和可重复的生物-（14）C-AMS 分析，样品制备中的石墨化步骤是最关键的步骤。因此，已经针对特定应用报告了各种用于称为石墨化的过程的样品制备方法。使用火焰密封硼硅酸盐管或隔垫密封小瓶的催化石墨化是生物-（14）C-AMS 的一种流行的样品制备方法。在这篇综述中，我们介绍了 AMS 系统，特别是用于生物-（14）C-AMS 的 AMS 系统。此外，我们还回顾了生物-（14）C-AMS 的石墨化方法，以促进对该技术样品制备的进一步理解和改进。描述了过去二十年来催化石墨化方法的示例。
