Tao Xiao-Yu, Zhang De-Wu, Chen Ri-Dao, Yin Yun-Ze, Zou Jian-Hua, Xie Dan, Yang Lin, Wang Chun-Mei, Dai Jun-Gui
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3738-42.
The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on physico-chemical properties and spectroscopic data, their structures were identified as isobavachalcone (1), genistein (2), norartocarpetin (3), albanin A (4), guangsangon E (5), mulberrofuran F (6), chalcomoracin (7), kuwanon J (8). Compounds 3-6 were isolated from the cell cultures of M. alba for the first time.
采用硅胶柱色谱、葡聚糖凝胶LH - 20柱色谱和半制备高效液相色谱法，从白桑培养基的乙酸乙酯提取物和细胞培养物的甲醇提取物中分离纯化化合物。分离得到8个化合物。根据理化性质和光谱数据，鉴定其结构分别为异补骨脂查耳酮（1）、染料木素（2）、去甲波罗蜜黄素（3）、白桑素A（4）、光桑素E（5）、桑呋喃F（6）、查耳酮莫拉辛（7）、桑酮J（8）。化合物3 - 6首次从白桑细胞培养物中分离得到。
