Sports Genetics Laboratory, St Petersburg Research Inst of Physical Culture, St Petersburg, Russia.
Int J Sports Physiol Perform. 2014 Jan;9(1):173-80. doi: 10.1123/ijspp.2013-0026. Epub 2013 Apr 23.
In red skeletal muscle, monocarboxylate transporter 1 (MCT1) is required for lactate to enter the myocytes for oxidation. The A1470T polymorphism (rs1049434) in the MCT1 gene was shown to be associated with lactate transport rates in human skeletal muscles. The aim of the study was to compare genotype and allele frequencies of the MCT1 gene polymorphism in 323 Russian athletes and 467 nonathletic controls and to investigate the association of the MCT1 gene A1470T polymorphism with maximal oxygen consumption and maximal lactate concentration in rowers (n = 79).
Genotyping for the A1470T MCT1 polymorphism was performed by PCR-RFLP method. Physiological measurements of 79 Russian rowers of national competitive standard were determined during an incremental test to exhaustion on a rowing ergometer.
Frequencies of the A allele (71.8% vs 62.5%, P < .0001) and AA genotype (59.8% vs 39.4%, P < .0001) were significantly higher in endurance-oriented athletes (n = 142) than in the control group. Mean blood lactate concentration was higher in male rowers with the T allele (AT+TT 10.26 ± 1.89 mmol/L, AA 8.75 ± 1.69 mmol/L, P = .005).
MCT1 gene A1470T polymorphism is associated with endurance athlete status and blood lactate level after intensive exercise.
在红色骨骼肌中，单羧酸转运蛋白 1（MCT1）对于乳酸进入肌细胞进行氧化是必需的。MCT1 基因中的 A1470T 多态性（rs1049434）与人体骨骼肌中的乳酸转运率有关。本研究的目的是比较 323 名俄罗斯运动员和 467 名非运动员对照者 MCT1 基因多态性的基因型和等位基因频率，并研究 MCT1 基因 A1470T 多态性与赛艇运动员（n = 79）最大摄氧量和最大乳酸浓度的关系。
采用 PCR-RFLP 法检测 MCT1 基因 A1470T 多态性的基因型。对 79 名具有国家竞技标准的俄罗斯赛艇运动员进行递增至力竭的划船测功仪测试，以确定其生理测量值。
在以耐力为导向的运动员（n = 142）中，A 等位基因（71.8%比 62.5%，P <.0001）和 AA 基因型（59.8%比 39.4%，P <.0001）的频率明显高于对照组。携带 T 等位基因（AT+TT 10.26 ± 1.89 mmol/L，AA 8.75 ± 1.69 mmol/L，P =.005）的男性赛艇运动员的平均血乳酸浓度更高。
MCT1 基因 A1470T 多态性与耐力运动员状态和剧烈运动后血乳酸水平有关。
