Department of Sport, Physical Activity and Health, University of Basel, 4001 Basel, Switzerland.
Swiss Federal Institute of Sport Magglingen SFISM, 2532 Magglingen, Switzerland.
Genes (Basel). 2024 Jul 14;15(7):918. doi: 10.3390/genes15070918.
Polymorphism rs1049434 characterizes the nonsynonymous exchange of adenosine (A) by thymidine (T) in the gene for monocarboxylate transporter 1 (). We tested whether T-allele carriers of rs1049434 demonstrate increased accumulation of markers of metabolic strain.
Physically active, healthy, young male subjects (n = 22) conducted a power-matched one-legged cycling exercise to exhaustion. Metabolic substrates in capillary blood, selected metabolic compounds, and indices for the slow oxidative phenotype of vastus lateralis muscle were quantified in samples collected before and after exercise. The genotypes of the rs1049434 polymorphism were determined with polymerase chain reactions.
One-legged exercise affected the concentration of muscle metabolites entering the tricarboxylic acid cycle, such as acetyl-co-enzyme A (+448%) and acetyl-L-carnitine (+548%), muscle glycogen (-59%), and adenosine monophosphate (-39%), 30 min post-exercise. Exercise-related variability in the muscular concentration of glycogen, long-chain acyl co-enzyme As and a triglyceride, nicotinamide adenine dinucleotide (NADH), and adenosine monophosphate (AMP) interacted with rs1049434. T-allele carriers demonstrated a 39% lesser reduction in glycogen after exercise than non-carriers when NADH increased only in the non-carriers. Muscle lactate concentration was 150% higher, blood triacyl-glyceride concentration was 53% lower, and slow fiber percentage was 20% lower in T-allele carriers.
The observations suggest a higher anaerobic glycolytic strain during exhaustive exercise and a lowered lipid handling in T-allele non-carriers.
单羧酸转运蛋白 1（）基因中的核苷酸 rs1049434 表现为腺嘌呤（A）到胸腺嘧啶（T）的非同义替换，这种多态性被称为 T 等位基因。我们测试了 rs1049434 的 T 等位基因携带者是否表现出代谢应激标志物的积累增加。
22 名健康的年轻男性进行了单侧腿蹬踏运动至力竭。在运动前后采集的毛细血管血液中测定代谢底物、选定的代谢物以及股外侧肌慢氧化表型的指标。使用聚合酶链反应确定 rs1049434 多态性的基因型。
单侧运动影响了进入三羧酸循环的肌肉代谢物浓度，如乙酰辅酶 A（+448%）和乙酰肉碱（+548%）、肌肉糖原（-59%）和腺苷一磷酸（-39%），运动后 30 分钟。运动相关的肌肉糖原、长链酰基辅酶 A 和甘油三酯、烟酰胺腺嘌呤二核苷酸（NADH）和腺苷一磷酸（AMP）的变化与 rs1049434 相互作用。当仅在非携带者中增加 NADH 时，T 等位基因携带者的糖原减少量比非携带者少 39%。T 等位基因携带者的肌肉乳酸浓度高 150%，血液三酰甘油浓度低 53%，慢肌纤维百分比低 20%。
这些观察结果表明，在剧烈运动中 T 等位基因非携带者的无氧糖酵解应激增加，脂质处理能力降低。
