CRISPR-Cas 系统在蓝藻门中的广泛分布证据。
Evidence for the widespread distribution of CRISPR-Cas system in the Phylum Cyanobacteria.
机构信息
U.S. Department of Energy-Joint Genome Institute, Walnut Creek, CA, USA.
出版信息
RNA Biol. 2013 May;10(5):687-93. doi: 10.4161/rna.24571. Epub 2013 Apr 11.
Abstract
Members of the phylum Cyanobacteria inhabit ecologically diverse environments. However, the CRISPR-Cas (clustered regularly interspaced short palindromic repeats, CRISPR associated genes), an extremely adaptable defense system, has not been surveyed in this phylum. We analyzed 126 cyanobacterial genomes and, surprisingly, found CRISPR-Cas in the majority except the marine subclade (Synechococcus and Prochlorococcus), in which cyanophages are a known force shaping their evolution. Multiple observations of CRISPR loci in the absence of cas1/cas2 genes may represent an early stage of losing a CRISPR-Cas locus. Our findings reveal the widespread distribution of their role in the phylum Cyanobacteria and provide a first step to systematically understanding CRISPR-Cas systems in cyanobacteria.
摘要
蓝藻门的成员栖息在生态多样的环境中。然而，CRISPR-Cas（成簇的规律间隔短回文重复序列，CRISPR 相关基因）是一种极其适应性强的防御系统，在这个门中尚未进行调查。我们分析了 126 个蓝藻基因组，令人惊讶的是，除了海洋亚群（聚球藻和原绿球藻）之外，大多数蓝藻都发现了 CRISPR-Cas，而在这些亚群中，噬藻体是塑造它们进化的已知力量。在没有 cas1/cas2 基因的情况下，CRISPR 基因座的多次观察可能代表失去 CRISPR-Cas 基因座的早期阶段。我们的发现揭示了它们在蓝藻门中的广泛分布，并为系统地了解蓝藻中的 CRISPR-Cas 系统提供了第一步。
相似文献
1
Evidence for the widespread distribution of CRISPR-Cas system in the Phylum Cyanobacteria.CRISPR-Cas 系统在蓝藻门中的广泛分布证据。
RNA Biol. 2013 May;10(5):687-93. doi: 10.4161/rna.24571. Epub 2013 Apr 11.
2
On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires.关于使用逆转录酶的CRISPR-Cas系统的起源及其超多样、神秘的间隔序列库
mBio. 2017 Jul 11;8(4):e00897-17. doi: 10.1128/mBio.00897-17.
3
Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Are Required for Spacer Acquisition.Cas1 和 Cas2 来自 的 II-C 型 CRISPR-Cas 系统，对于间隔区获取是必需的。
Front Cell Infect Microbiol. 2018 Jun 12;8:195. doi: 10.3389/fcimb.2018.00195. eCollection 2018.
4
CRISPR-Cas systems in multicellular cyanobacteria.多细胞蓝细菌中的 CRISPR-Cas 系统。
RNA Biol. 2019 Apr;16(4):518-529. doi: 10.1080/15476286.2018.1493330. Epub 2018 Aug 15.
5
Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis.通过基因邻域分析系统地预测与 CRISPR-Cas 系统功能相关的基因。
Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5307-E5316. doi: 10.1073/pnas.1803440115. Epub 2018 May 21.
6
How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.II型CRISPR-Cas如何通过Cas1-Cas2介导的间隔序列整合建立免疫。
Nature. 2017 Oct 5;550(7674):137-141. doi: 10.1038/nature24020. Epub 2017 Sep 4.
7
CRISPR-Cas: evolution of an RNA-based adaptive immunity system in prokaryotes.CRISPR-Cas：原核生物中基于 RNA 的适应性免疫系统的进化。
RNA Biol. 2013 May;10(5):679-86. doi: 10.4161/rna.24022. Epub 2013 Feb 25.
8
Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Reveals Novel Insights into Virus-Bacterium Interactions.基因组特征分析表明，感染丝状固氮蓝藻的噬藻体 vB_AphaS-CL131 揭示了病毒-细菌相互作用的新见解。
Appl Environ Microbiol. 2018 Dec 13;85(1). doi: 10.1128/AEM.01311-18. Print 2019 Jan 1.
9
Adaptation and modification of three CRISPR loci in two closely related cyanobacteria.在两种密切相关的蓝藻中对三个 CRISPR 基因座进行适应性改造和修饰。
RNA Biol. 2013 May;10(5):852-64. doi: 10.4161/rna.24160. Epub 2013 Mar 27.
10
Viruses Infecting a Freshwater Filamentous Cyanobacterium (Nostoc sp.) Encode a Functional CRISPR Array and a Proteobacterial DNA Polymerase B.感染一种淡水丝状蓝细菌（念珠藻属）的病毒编码一个功能性CRISPR阵列和一个变形菌DNA聚合酶B。
mBio. 2016 Jun 14;7(3):e00667-16. doi: 10.1128/mBio.00667-16.
引用本文的文献
1
Cyanophage Infections in a Sponge Intracellular Cyanobacterial Symbiont.海绵细胞内蓝藻共生体中的噬蓝藻体感染
Environ Microbiol. 2025 Jul;27(7):e70155. doi: 10.1111/1462-2920.70155.
2
Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications.蓝藻I型CRISPR-Cas系统：分布、机制及基因组编辑应用
Front Bioeng Biotechnol. 2025 Feb 27;13:1552030. doi: 10.3389/fbioe.2025.1552030. eCollection 2025.
3
Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System.I型CRISPR-Cas系统的作用机制及应用研究进展
Int J Mol Sci. 2024 Nov 22;25(23):12544. doi: 10.3390/ijms252312544.
4
Uncovering the genomic basis of symbiotic interactions and niche adaptations in freshwater picocyanobacteria.揭示淡水微囊藻共生相互作用和生态位适应的基因组基础。
Microbiome. 2024 Aug 10;12(1):150. doi: 10.1186/s40168-024-01867-0.
5
Microbial diversity, genomics, and phage-host interactions of cyanobacterial harmful algal blooms.蓝藻有害藻华的微生物多样性、基因组学和噬菌体-宿主相互作用。
mSystems. 2024 Jul 23;9(7):e0070923. doi: 10.1128/msystems.00709-23. Epub 2024 Jun 10.
6
Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects.成簇规律间隔短回文重复序列/CRISPR相关蛋白及其在海洋领域的应用：现状、挑战与前景
Microorganisms. 2024 Jan 6;12(1):118. doi: 10.3390/microorganisms12010118.
7
Genomic insights into the adaptation of to the coastal environment on Xiamen.关于[具体研究对象]对厦门沿海环境适应性的基因组学见解。 （注：原文中“of to”表述有误，推测是“of [具体研究对象] to”，这里按推测的正确形式翻译了，如果有准确的原文修正信息，可进一步完善译文）
Front Microbiol. 2023 Nov 20;14:1292150. doi: 10.3389/fmicb.2023.1292150. eCollection 2023.
8
Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake.从淡水湖中宏基因组组装的七个噬菌体基因组的基因组分析和分类学特征
Viruses. 2023 Sep 30;15(10):2038. doi: 10.3390/v15102038.
9
The biology and type I/III hybrid nature of type I-D CRISPR-Cas systems.I 型-D CRISPR-Cas 系统的生物学和 I/III 型混合性质。
Biochem J. 2023 Apr 13;480(7):471-488. doi: 10.1042/BCJ20220073.
10
Molecular evidence of parallel evolution in a cyanophage.分子证据表明噬藻体呈平行进化。
PLoS One. 2023 Feb 9;18(2):e0281537. doi: 10.1371/journal.pone.0281537. eCollection 2023.
本文引用的文献
1
Adaptation and modification of three CRISPR loci in two closely related cyanobacteria.在两种密切相关的蓝藻中对三个 CRISPR 基因座进行适应性改造和修饰。
RNA Biol. 2013 May;10(5):852-64. doi: 10.4161/rna.24160. Epub 2013 Mar 27.
2
CRISPR-Cas systems in the cyanobacterium Synechocystis sp. PCC6803 exhibit distinct processing pathways involving at least two Cas6 and a Cmr2 protein.集胞藻 PCC6803 中的 CRISPR-Cas 系统表现出不同的加工途径，涉及至少两种 Cas6 和一种 Cmr2 蛋白。
PLoS One. 2013;8(2):e56470. doi: 10.1371/journal.pone.0056470. Epub 2013 Feb 18.
3
Comparative genomic analysis of phylogenetically closely related Hydrogenobaculum sp. isolates from Yellowstone National Park.黄石国家公园中系统发育关系密切的嗜氢菌属菌株的比较基因组分析。
Appl Environ Microbiol. 2013 May;79(9):2932-43. doi: 10.1128/AEM.03591-12. Epub 2013 Feb 22.
4
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.利用 Cas9 RNA 引导的内切酶在人类细胞中进行靶向基因组工程。
Nat Biotechnol. 2013 Mar;31(3):230-2. doi: 10.1038/nbt.2507. Epub 2013 Jan 29.
5
RNA-guided editing of bacterial genomes using CRISPR-Cas systems.利用 CRISPR-Cas 系统对细菌基因组进行 RNA 引导编辑。
Nat Biotechnol. 2013 Mar;31(3):233-9. doi: 10.1038/nbt.2508. Epub 2013 Jan 29.
6
Efficient genome editing in zebrafish using a CRISPR-Cas system.利用 CRISPR-Cas 系统在斑马鱼中进行高效的基因组编辑。
Nat Biotechnol. 2013 Mar;31(3):227-9. doi: 10.1038/nbt.2501. Epub 2013 Jan 29.
7
RNA-guided human genome engineering via Cas9.通过 Cas9 进行 RNA 引导的人类基因组工程。
Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033. Epub 2013 Jan 3.
8
Multiplex genome engineering using CRISPR/Cas systems.利用 CRISPR/Cas 系统进行多重基因组工程。
Science. 2013 Feb 15;339(6121):819-23. doi: 10.1126/science.1231143. Epub 2013 Jan 3.
9
Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing.利用多样性驱动的基因组测序提高蓝藻门的覆盖率。
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1053-8. doi: 10.1073/pnas.1217107110. Epub 2012 Dec 31.
10
Viral diversity threshold for adaptive immunity in prokaryotes.原核生物中适应性免疫的病毒多样性阈值。
mBio. 2012 Dec 4;3(6):e00456-12. doi: 10.1128/mBio.00456-12.
Zotero 插件