College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China.
Viruses. 2023 Sep 30;15(10):2038. doi: 10.3390/v15102038.
Viruses in aquatic ecosystems exhibit remarkable abundance and diversity. However, scattered studies have been conducted to mine uncultured viruses and identify them taxonomically in lake water. Here, whole genomes (29-173 kbp) of seven uncultured dsDNA bacteriophages were discovered in Dishui Lake, the largest artificial lake in Shanghai. We analyzed their genomic signatures and found a series of viral auxiliary metabolic genes closely associated with protein synthesis and host metabolism. Dishui Lake phages shared more genes with uncultivated environmental viruses than with reference viruses based on the gene-sharing network classification. Phylogeny of proteomes and comparative genomics delineated three new genera within two known viral families of and , and four new families in for these seven novel phages. Their potential hosts appeared to be from the dominant bacterial phyla in Dishui Lake. Altogether, our study provides initial insights into the composition and diversity of bacteriophage communities in Dishui Lake, contributing valuable knowledge to the ongoing research on the roles played by viruses in freshwater ecosystems.
水生生态系统中的病毒具有显著的丰度和多样性。然而，目前只有少量研究致力于挖掘未培养的病毒并对其进行分类学鉴定。本研究在上海最大的人工湖——滴水湖发现了 7 种未培养的 dsDNA 噬菌体的全基因组（29-173 kbp）。我们分析了它们的基因组特征，发现了一系列与蛋白质合成和宿主代谢密切相关的病毒辅助代谢基因。基于基因共享网络分类，与参考病毒相比，滴水湖噬菌体与未培养的环境病毒共享更多的基因。基于蛋白质组的系统发育和比较基因组学，将这 7 种新型噬菌体划分为 2 个已知病毒科（ 和 ）中的 3 个新属，以及 4 个新科。它们的潜在宿主似乎来自于滴水湖中占优势的细菌门。总之，本研究初步揭示了滴水湖噬菌体群落的组成和多样性，为正在进行的病毒在淡水生态系统中所扮演的角色的研究提供了有价值的知识。
