Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University, School of Medicine, PO Box 19679, 701 North First Street, Springfield, Illinois, 62794-9679, USA.
J Orthop Res. 2013 Sep;31(9):1374-81. doi: 10.1002/jor.22379. Epub 2013 Apr 29.
The purpose of this study was to develop single walled carbon nanotubes (SWCNT) and poly lactic-co-glycolic acid (PLAGA) composites for orthopedic applications and to evaluate the interaction of human stem cells (hBMSCs) and osteoblasts (MC3T3-E1 cells) via cell growth, proliferation, gene expression, extracellular matrix production and mineralization. PLAGA and SWCNT/PLAGA composites were fabricated with various amounts of SWCNT (5, 10, 20, 40, and 100 mg), characterized and degradation studies were performed. Cells were seeded and cell adhesion/morphology, growth/survival, proliferation and gene expression analysis were performed to evaluate biocompatibility. Imaging studies demonstrated uniform incorporation of SWCNT into the PLAGA matrix and addition of SWCNT did not affect the degradation rate. Imaging studies revealed that MC3T3-E1 and hBMSCs cells exhibited normal, non-stressed morphology on the composites and all were biocompatible. Composites with 10 mg SWCNT resulted in highest rate of cell proliferation (p < 0.05) among all composites. Gene expression of alkaline phosphatase, collagen I, osteocalcin, osteopontin, Runx-2, and Bone Sialoprotein was observed on all composites. In conclusion, SWCNT/PLAGA composites imparted beneficial cellular growth capabilities and gene expression, and mineralization abilities were well established. These results demonstrate the potential of SWCNT/PLAGA composites for musculoskeletal regeneration and bone tissue engineering (BTE) and are promising for orthopedic applications.
本研究旨在开发用于骨科应用的单壁碳纳米管 (SWCNT) 和聚乳酸-共-羟基乙酸 (PLAGA) 复合材料，并通过细胞生长、增殖、基因表达、细胞外基质产生和矿化来评估人干细胞 (hBMSCs) 和成骨细胞 (MC3T3-E1 细胞) 之间的相互作用。用不同量的 SWCNT（5、10、20、40 和 100mg）制备了 PLAGA 和 SWCNT/PLAGA 复合材料，并对其进行了表征和降解研究。接种细胞并进行细胞粘附/形态、生长/存活、增殖和基因表达分析，以评估生物相容性。成像研究表明 SWCNT 均匀地掺入到 PLAGA 基质中，并且添加 SWCNT 不会影响降解速率。成像研究表明，MC3T3-E1 和 hBMSCs 细胞在复合材料上表现出正常、无应激的形态，所有细胞均具有生物相容性。在所有复合材料中，含 10mgSWCNT 的复合材料具有最高的细胞增殖率（p<0.05）。所有复合材料均观察到碱性磷酸酶、胶原 I、骨钙素、骨桥蛋白、Runx-2 和骨涎蛋白的基因表达。总之，SWCNT/PLAGA 复合材料赋予细胞生长能力和基因表达有益的影响，并且矿化能力得到了很好的证实。这些结果表明 SWCNT/PLAGA 复合材料在肌肉骨骼再生和骨组织工程（BTE）方面具有潜力，有望用于骨科应用。
