小窝介导的共轭聚合物纳米颗粒内吞作用。
Caveolae-mediated endocytosis of conjugated polymer nanoparticles.
机构信息
Department of Chemistry, Florida International University, Miami, FL 33199, USA.
出版信息
Macromol Biosci. 2013 Jul;13(7):913-20. doi: 10.1002/mabi.201300030. Epub 2013 Apr 30.
Abstract
Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME is further confirmed by high co-localization with caveolin-1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.
摘要
了解合成生物材料的细胞进入途径对于提高整体标记和输送效率非常重要。本文介绍了共轭聚合物纳米粒子（CPNs）的细胞进入机制。CPNs 是用于各种生物应用的固有荧光材料。虽然 CPNs 没有毒性，但用 caveolae 介导的内吞作用（CvME）抑制剂染料木黄酮预处理的癌细胞中观察到 CPNs 的摄取减少。CvME 进一步通过与在小窝和 caveosomes 中发现的 caveolin-1 蛋白的高共定位得到证实。出色的光物理性质、非毒性和非破坏性的输送途径表明，CPNs 是有前途的多功能载体，可以最大程度地减少输送过程中内容物的降解。
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