Koroleva Iu V, Minenkova I B, Shamshina T N, Azizbekian R R
Mol Gen Mikrobiol Virusol. 1993 Jan-Feb(1):35-8.
A new bacteriophage Tt91 of larger size and broad lytical spectrum has been isolated from soil to widen the possibility to study new Bacillus thuringiensis strains. The Tt91 bacteriophage was shown to perform the intravariant transduction and, thus, can be used for genetic mapping. The ultrastructural analysis made it possible to refer bacteriophage Tt91 to morphotype A1 and showed similarity of Tt91 morphological structure to the one of CP-group bacteriophages. The performed comparative analysis of phage-specific proteins has confirmed a possible relation of the bacteriophages and revealed the differences between them. The data on the resistance of Tt91 DNA to majority of site-specific restriction endonucleases (17 out of 18 tested) and on DNA melting in the wide interval of temperatures suggests the presence of possible anomalies in the Tt91 DNA.
从土壤中分离出一种新的、尺寸更大且裂解谱更广的噬菌体Tt91，以拓宽研究新型苏云金芽孢杆菌菌株的可能性。Tt91噬菌体被证明可进行变体间转导，因此可用于基因图谱绘制。超微结构分析使噬菌体Tt91可归为A1形态型，并显示Tt91的形态结构与CP组噬菌体之一相似。对噬菌体特异性蛋白质进行的比较分析证实了噬菌体之间可能存在的关系，并揭示了它们之间的差异。关于Tt91 DNA对大多数位点特异性限制性内切酶（18种测试酶中的17种）具有抗性以及在很宽的温度区间内DNA熔解的数据表明，Tt91 DNA可能存在异常。
