大鼠冠状病毒和5株涎泪腺炎病毒在大鼠细胞系LBC细胞中的蚀斑测定及增殖

Plaque assay and propagation in rat cell line LBC cells of rat coronavirus and 5 strains of sialodacryoadenitis virus.

作者信息

Hirano N

机构信息

Department of Veterinary Microbiology, Iwate University, Morioka, Japan.

出版信息

Zentralbl Veterinarmed B. 1990 Mar;37(2):91-6. doi: 10.1111/j.1439-0450.1990.tb01031.x.

DOI:10.1111/j.1439-0450.1990.tb01031.x

PMID:2363328

Abstract

Various factors influencing the plaque formation of rat coronavirus (RCV) and sialodacryoadenitis virus (SDAV) in LBC cell monolayers were studied to develop the practical method for plaque assay. By this method, 4 Japanese isolates of SDAV also produced clear plaques. In one-step growth experiments of these viruses, newly formed virus was first recognized within 7.5 h postinfection and showed subsequently a rapid exponential increase. The virus was released rapidly from the infected cells. By indirect immunofluorescence virus specific antigen was detected as perinuclear granules in the cytoplasm of the cells at 5-6 h postinfection, and all the cells revealed fluorescence at 12 h postinfection.

摘要

研究了影响大鼠冠状病毒（RCV）和涎泪腺炎病毒（SDAV）在LBC细胞单层中形成蚀斑的各种因素，以开发蚀斑测定的实用方法。通过这种方法，4株日本SDAV分离株也产生了清晰的蚀斑。在这些病毒的一步生长实验中，新形成的病毒在感染后7.5小时内首次被识别，随后呈快速指数增长。病毒从感染细胞中迅速释放。通过间接免疫荧光法，在感染后5-6小时，病毒特异性抗原在细胞质中被检测为核周颗粒，在感染后12小时所有细胞均显示荧光。