Department of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implant, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2010 Oct;95(1):192-201. doi: 10.1002/jbm.b.31702.
The CaO-ZrO2-SiO2 (CZS) coating was prepared by plasma-spraying chemically synthesized CZS powder onto a Ti-6Al-4V substrate. This CZS coating has been demonstrated to have good bioactivity, high bonding strength with the substrate and a low degradation rate. However, the effect of CZS coating on the osseointegration of bone-implant is still unknown. In this study, human bone marrow-derived stromal cells (hBMSCs) were cultured on CZS coating in vitro, and cell behavior was investigated, with the classical hydroxyapatite (HA) coating as a control. Scanning electron microscopy (SEM) and immunofluorescence studies showed that the hBMSCs on the CZS coating spread well with organized cytoskeleton structure at 24 h following cell seeding. The MTT assay and the Alamar Blue assay indicated that CZS coating promoted the attachment and proliferation of hBMSCs. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes, such as ALP, collagen I (COLI), osteopontin (OPN), and osteocalcin (OCN), demonstrated that the osteoblastic differentiation of hBMSCs was enhanced more by CZS coating than by HA coating. These results suggest that CZS coating possess excellent biological properties and may have potential in biomedical applications.
采用等离子喷涂技术，将化学合成的 CaO-ZrO2-SiO2（CZS）粉末喷涂在 Ti-6Al-4V 基底上，制备出 CZS 涂层。该 CZS 涂层具有良好的生物活性、与基底较高的结合强度和较低的降解速率。然而，CZS 涂层对骨-植入体骨整合的影响尚不清楚。本研究采用人骨髓基质细胞（hBMSCs）在体外培养于 CZS 涂层上，以经典的羟基磷灰石（HA）涂层作为对照，研究细胞行为。扫描电子显微镜（SEM）和免疫荧光研究表明，细胞接种后 24 h，hBMSCs 在 CZS 涂层上伸展良好，细胞骨架结构有序。MTT 检测和 Alamar Blue 检测表明，CZS 涂层促进了 hBMSCs 的黏附和增殖。碱性磷酸酶（ALP）活性检测和骨形成标志物基因（如 ALP、胶原 I（COLI）、骨桥蛋白（OPN）和骨钙素（OCN））的表达结果表明，CZS 涂层对 hBMSCs 的成骨分化的促进作用强于 HA 涂层。这些结果表明，CZS 涂层具有优异的生物学性能，可能在生物医学应用中有潜在的应用价值。
