Department for Farm Animals and Veterinary Public Health, Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna 1210, Austria.
Vet Rec. 2013 May 25;172(21):556. doi: 10.1136/vr.101262. Epub 2013 May 1.
Numerous cases of tenosynovitis appeared in France causing high morbidity in free-range and standard broilers. The main clinical findings were lameness, stunting and non-uniform bodyweights. Although the natural mortality was low, the economic losses due to birds that had to be removed from the flock prematurely, downgrading of carcases and lower average weights at slaughter were substantial. Postmortem examinations, bacteriological, virological and serological examination confirmed the aetiology of avian orthoreovirus (ARV)-induced tenosynovitis. The isolated ARVs were analysed serologically and genetically. Sequencing of σC RT-PCR products and phylogenetic analysis revealed a new type of ARV. The virus was not neutralised in serum neutralisation test using monovalent sera from vaccinated chickens. Together with the flock data, epidemiology of these recent reovirus outbreaks in France was reconstructed. It is concluded that these reovirus isolates differ serologically and genetically from the well described reovirus isolates used in commercial vaccines which were not capable of preventing the disease. The outbreaks resulted in substantial losses in broilers from vaccinated breeders.
法国出现了许多腱鞘炎病例，导致自由放养和标准肉鸡的发病率很高。主要的临床发现是跛行、发育不良和体重不均匀。尽管自然死亡率很低，但由于必须提前从鸡群中淘汰、降级和屠宰平均体重较低的鸡，造成了巨大的经济损失。剖检、细菌学、病毒学和血清学检查证实了禽正呼肠孤病毒（ARV）引起的腱鞘炎的病因。分离的 ARV 进行了血清学和遗传学分析。σC RT-PCR 产物的测序和系统发育分析显示出一种新型 ARV。血清中和试验中，用接种疫苗鸡的单价血清不能中和该病毒。结合农场数据，重建了法国最近这些呼肠孤病毒暴发的流行病学。结论是，这些呼肠孤病毒分离株在血清学和遗传学上与商业疫苗中使用的描述良好的呼肠孤病毒分离株不同，商业疫苗不能预防这种疾病。这些暴发导致接种种鸡的肉鸡大量损失。
