Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.
Mol Biotechnol. 2013 Oct;55(2):111-9. doi: 10.1007/s12033-013-9661-9.
Induced multipotent stem (iMS) cells are originated from somatic cells and become multipotent by genetic and/or epigenetic modifications. Previous studies have shown that the fish oocytes extracts (FOE) can induce skin fibroblast cells into iMS cells. In this study, we aim to determine whether FOE can similarly induce mouse peripheral blood mononuclear cells (PBMCs) into the iMS state and if so, whether they can survive longer when they are transplanted into the irradiation female mice. PBMCs of GFP-transgenic male mice were cultured and transiently reprogrammed by FOE. They were deemed reaching the iMS state after detection of expression of stem cell markers. The iMS-like PBMCs were transplanted into female C57BL mice by tail vein injection. The spleen wet weights as well as numbers of colonies of the recipient mice were examined. The results showed the spleen wet weights and numbers of spleen colonies of FOE-induced group were all significantly higher than those of the non-induced group and negative control group. On day 90 after transplantation, FISH analysis detected the presence of Y chromosome in the induced group, but not of the other groups. The current findings demonstrate that FOE-induced PBMCs are able to survive longer in irradiated female mice.
诱导多能干细胞（iMS）来源于体细胞，通过遗传和/或表观遗传修饰成为多能性的。先前的研究表明，鱼卵提取物（FOE）可以诱导皮肤成纤维细胞成为 iMS 细胞。在这项研究中，我们旨在确定 FOE 是否可以类似地将小鼠外周血单核细胞（PBMC）诱导至 iMS 状态，如果可以，它们在被移植到照射的雌性小鼠中时是否能够存活更长时间。GFP 转基因雄性小鼠的 PBMC 经 FOE 培养并短暂重编程。在检测到干细胞标志物的表达后，它们被认为达到了 iMS 状态。iMS 样 PBMC 通过尾静脉注射到 C57BL 雌性小鼠中。检查受体小鼠的脾脏湿重和集落数量。结果表明，FOE 诱导组的脾脏湿重和脾脏集落数量均明显高于未诱导组和阴性对照组。移植后 90 天，FISH 分析在诱导组中检测到 Y 染色体的存在，但在其他组中未检测到。目前的研究结果表明，FOE 诱导的 PBMC 在照射的雌性小鼠中能够存活更长时间。
