通过RNA-PAGE和RT-PCR检测牛犊中的轮状病毒感染
Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR.
作者信息
Basera Sanjay Singh, Singh Rashmi, Vaid N, Sharma K, Chakravarti S, Malik Y P S
机构信息
Department of Veterinary Microbiology, College of Veterinary and Animal Science, G. B. Pant University of Agriculture and Technology, Pantnagar, U. S. Nagar, Uttarakhand India.
出版信息
Indian J Virol. 2010 Oct;21(2):144-7. doi: 10.1007/s13337-010-0017-9. Epub 2011 Feb 7.
Abstract
A total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from Pantnagar and Dehradun during winter months. Of the 110 cattle calves screened by RNA-PAGE, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. All samples were found to have long electropherotype and two distinct electropherotypes having segment variation were observed. The overall prevalence of rotavirus was 10.15% (13/128). RT-PCR targeting group specific VP6 gene confirmed Group A rotavirus in 10 out of 13 samples, while three samples remained un-groupable.
摘要
在冬季，从潘特纳加尔和德拉敦的牛犊和水牛犊中总共采集了128份腹泻粪便样本。在通过RNA-PAGE筛查的110头小牛犊中，13份样本（11.81%）检测到轮状病毒，而水牛犊的样本未发现阳性。所有样本均具有长电泳型，观察到两种具有片段变异的不同电泳型。轮状病毒的总体患病率为10.15%（13/128）。针对组特异性VP6基因的RT-PCR在13份样本中的10份中确认了A组轮状病毒，而3份样本无法分型。
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