Greenlee P G, Filippa D A, Quimby F W, Patnaik A K, Calvano S E, Matus R E, Kimmel M, Hurvitz A I, Lieberman P H
Department of Pathology, Cornell University Medical College, New York.
Cancer. 1990 Aug 1;66(3):480-90. doi: 10.1002/1097-0142(19900801)66:3<480::aid-cncr2820660314>3.0.co;2-x.
One hundred seventy-six canine lymphomas were classified morphologically using four of the major human lymphoma classification schemes (Rappaport, Lukes-Collins, Kiel, and the Working Formulation). All 176 dogs received the same chemotherapeutic protocol. Sixty-two of these lymphomas had their immunophenotypes established by examination of cell surface markers by automated cytofluorography. Several different morphologic types of canine lymphoma were identified and these were comparable to morphologic categories in human classification schemes. Follicular and low grade lymphomas were rare. The two most common morphologic types were diffuse large cell (centroblastic) and immunoblastic. The Kiel classification appeared to be the most useful human scheme for classifying the canine lymphomas. Cytofluorographic analysis was generally straightforward, and 60 of the 62 lymphomas were placed into one of three immunophenotypic categories: 27 pan-T(LQ1)+SIg+, 21 pan-T(LQ1)-SIg+, and 12 pan-T(LQ1)+SIg-. Two of the lymphomas could not be characterized immunologically because a pre-existing or reactive non-neoplastic population of lymphocytes made interpretation of single cell suspension analysis difficult. The authors identified correlations between morphology and survival and disease-free remission; dogs with high-grade tumors generally survived the longest and had the longest remissions. No correlations were identified between high concentrations of serum lactate dehydrogenase, age, sex, or stage of disease, and morphology, immunophenotype, remission, or survival times. A significant correlation between clinical illness and survival time was documented. The median age of the dogs was nine years, no significant effect of sex on prevalence was observed, and some breeds were significantly overrepresented. Significant morphologic-immunophenotypic correlations included shorter remission and survival times for T-cell tumors than B-cell tumors, and a highly significant correlation between the pan-T(LQ1)+SIg-"T cell" phenotype and hypercalcemia.
使用四种主要的人类淋巴瘤分类方案（拉帕波特分类法、卢克斯 - 柯林斯分类法、基尔分类法和工作分类法）对176例犬淋巴瘤进行形态学分类。所有176只犬均接受相同的化疗方案。其中62例淋巴瘤通过自动细胞荧光术检测细胞表面标志物确定其免疫表型。识别出几种不同形态类型的犬淋巴瘤，这些与人类分类方案中的形态学类别相似。滤泡性和低级别淋巴瘤很少见。两种最常见的形态学类型是弥漫性大细胞（中心母细胞性）和免疫母细胞性。基尔分类法似乎是对犬淋巴瘤进行分类最有用的人类分类方案。细胞荧光分析通常很简单，62例淋巴瘤中有60例被归入三种免疫表型类别之一：27例全T（LQ1）+SIg +、21例全T（LQ1）-SIg +和12例全T（LQ1）+SIg -。其中2例淋巴瘤无法进行免疫学特征描述，因为预先存在的或反应性的非肿瘤性淋巴细胞群体使得单细胞悬液分析的解读变得困难。作者确定了形态学与生存及无病缓解之间的相关性；高级别肿瘤的犬通常存活时间最长且缓解期最长。未发现血清乳酸脱氢酶高浓度、年龄、性别或疾病分期与形态学、免疫表型、缓解或生存时间之间存在相关性。记录了临床疾病与生存时间之间的显著相关性。犬的中位年龄为9岁，未观察到性别对患病率有显著影响，且某些品种的比例明显过高。显著的形态学 - 免疫表型相关性包括T细胞肿瘤的缓解期和生存时间比B细胞肿瘤短，以及全T（LQ1）+SIg -“T细胞”表型与高钙血症之间高度显著的相关性。
