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八聚体是人类着丝粒处 CENP-A 核小体的主要形式。

The octamer is the major form of CENP-A nucleosomes at human centromeres.

机构信息

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

出版信息

Nat Struct Mol Biol. 2013 Jun;20(6):687-95. doi: 10.1038/nsmb.2562. Epub 2013 May 5.

DOI:10.1038/nsmb.2562
PMID:23644596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3760417/
Abstract

The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171-base-pair monomers at normal centromeres and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA-wrapping behavior, as do octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on conventional nucleosomes containing canonical histone H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini.

摘要

着丝粒是确保细胞分裂过程中基因组传递保真度的染色体位置。着丝粒蛋白 A (CENP-A) 是一种组蛋白 H3 变体，它独立于 DNA 序列指定着丝粒位置。关于 CENP-A 核小体的组蛋白组成和化学计量学，出现了相互矛盾的证据。在这里，我们表明在人类着丝粒上，CENP-A 核小体的主要形式是八聚体核小体。在正常着丝粒上，CENP-A 核小体高度有序地与α-卫星 171 碱基对单体结合，并且在新着丝粒上也显示出强烈的定位。在任何一种功能着丝粒上，CENP-A 核小体都表现出类似的 DNA 包装行为，与用重组成分重建的八聚体 CENP-A 核小体一样，其 DNA 末端比含有经典组蛋白 H3 的常规核小体松散。因此，在哺乳动物中，表观遗传指定着丝粒位置的染色质的基本单位是具有松散末端的八聚体核小体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/ab27915dff5f/nihms453767f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/a1b4022f4cc7/nihms453767f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/45fb53140abf/nihms453767f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/ef7e8689f2aa/nihms453767f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/b92e82ad62cd/nihms453767f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/ab27915dff5f/nihms453767f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/a1b4022f4cc7/nihms453767f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/d6c15ea69d54/nihms453767f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/4344bca46ea2/nihms453767f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/45fb53140abf/nihms453767f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/ef7e8689f2aa/nihms453767f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/b92e82ad62cd/nihms453767f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cc1/3760417/ab27915dff5f/nihms453767f7.jpg

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2
Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo.体内人源 CENP-A 核小体的细胞周期依赖性结构转变。
Cell. 2012 Jul 20;150(2):317-26. doi: 10.1016/j.cell.2012.05.035.
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Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeast.酵母着丝粒核小体的细胞周期偶联结构振荡。
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Plant Cell. 2025 Jul 1;37(7). doi: 10.1093/plcell/koaf142.
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