Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
Nat Struct Mol Biol. 2013 Jun;20(6):763-5. doi: 10.1038/nsmb.2574. Epub 2013 May 5.
Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
带有 CENP-A 替换的组蛋白 H3 的核小体直接指导动粒装配。与典型的八聚体 H3 核小体相比，已报道来自人类和果蝇的 CENP-A 核小体的高度降低，因此表明存在独特的四聚体半体组成。我们证明体外组装的八聚体 CENP-A 核小体表现出降低的高度，表明它们在物理上与 H3 核小体不同，并且否定了需要引入半体的需要。
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