CENP-A 赋予八聚体核小体降低的高度。
CENP-A confers a reduction in height on octameric nucleosomes.
机构信息
Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
出版信息
Nat Struct Mol Biol. 2013 Jun;20(6):763-5. doi: 10.1038/nsmb.2574. Epub 2013 May 5.
Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
带有 CENP-A 替换的组蛋白 H3 的核小体直接指导动粒装配。与典型的八聚体 H3 核小体相比，已报道来自人类和果蝇的 CENP-A 核小体的高度降低，因此表明存在独特的四聚体半体组成。我们证明体外组装的八聚体 CENP-A 核小体表现出降低的高度，表明它们在物理上与 H3 核小体不同，并且否定了需要引入半体的需要。
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