Pitts Katie L, Fenech Marianne
Department of Chemical and Biological Engineering, University of Ottawa.
J Vis Exp. 2013 Apr 25(74):e50314. doi: 10.3791/50314.
Micro-particle image velocimetry (μPIV) is used to visualize paired images of micro particles seeded in blood flows. The images are cross-correlated to give an accurate velocity profile. A protocol is presented for μPIV measurements of blood flows in microchannels. At the scale of the microcirculation, blood cannot be considered a homogeneous fluid, as it is a suspension of flexible particles suspended in plasma, a Newtonian fluid. Shear rate, maximum velocity, velocity profile shape, and flow rate can be derived from these measurements. Several key parameters such as focal depth, particle concentration, and system compliance, are presented in order to ensure accurate, useful data along with examples and representative results for various hematocrits and flow conditions.
微粒子图像测速技术（μPIV）用于可视化血流中植入的微粒的成对图像。这些图像通过互相关联以给出准确的速度分布。本文介绍了一种用于微通道内血流μPIV测量的方案。在微循环尺度下，血液不能被视为均匀流体，因为它是悬浮在牛顿流体血浆中的柔性颗粒的悬浮液。剪切速率、最大速度、速度分布形状和流速可从这些测量中得出。文中给出了几个关键参数，如焦深、颗粒浓度和系统顺应性，以确保获得准确、有用的数据，并给出了不同血细胞比容和流动条件下的示例及代表性结果。
