Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, China.
Acta Pharmacol Sin. 2013 Jul;34(7):983-8. doi: 10.1038/aps.2013.17. Epub 2013 May 6.
novel hemostatic sealant based on the in situ gel formation of gelatin catalyzed by thrombin and crosslinked by transglutaminase was designed. The aim of this study was to investigate the efficacy of the hemostatic sealant in control of bleeding caused by liver trauma in dogs.
Hepatic trauma that mimicked the grade III-IV rupture of liver was made in 20 dogs. The traumatic lesion was topically administered the hemostatic sealant (treatment group, n=10), or a thrombin solution (control group, n=10). The time to achieve hemostasis and the blood loss were measured. Contrast-enhanced ultrasound (CEUS) examination was performed in each animal on d 3, d 7, and d 10 d postoperatively to study the healing of the lesions.
The mean time to achieve hemostasis in the treatment group was significantly shorter than that in the control group (1.20±0.33 vs 6.70±0.64 min, P<0.05). The mean blood loss in the treatment group was significantly less than that in the control group (47.22±8.61 vs 79.29±11.97 mL, P<0.05). In CEUS examination, the traumatic lesions in the treatment group became significantly smaller on d 3, and disappeared on d 7, whereas the lesions in the control group still existed on d 10. Ascites were never found during 10 d follow-up in the treatment group but were observed on d 3 and d 7 in the control group.
Compared with thrombin, the novel hemostatic sealant shows much better efficacy in hemostasis and may promote wound healing in dog liver trauma.
设计一种新型止血密封剂，该密封剂基于凝血酶原位诱导明胶形成凝胶并通过转谷氨酰胺酶交联。本研究旨在探讨该止血密封剂控制犬肝脏创伤出血的效果。
在 20 只犬中模拟肝脏 III-IV 级破裂造成肝创伤。局部应用止血密封剂（治疗组，n=10）或凝血酶溶液（对照组，n=10）处理创伤病变。测量达到止血所需的时间和失血量。术后第 3、7 和 10 天，对每只动物进行超声造影（CEUS）检查，以研究病变的愈合情况。
治疗组达到止血的平均时间明显短于对照组（1.20±0.33 与 6.70±0.64 分钟，P<0.05）。治疗组的平均失血量明显少于对照组（47.22±8.61 与 79.29±11.97 毫升，P<0.05）。在 CEUS 检查中，治疗组的创伤病变在第 3 天明显缩小，第 7 天消失，而对照组在第 10 天仍存在病变。在治疗组的 10 天随访中从未发现腹水，但在对照组的第 3 和第 7 天观察到腹水。
与凝血酶相比，新型止血密封剂在止血方面具有更好的效果，可能促进犬肝脏创伤的伤口愈合。
