Department of Biomedical Engineering, the Institute for Nanobiotechnology, and the Translational Tissue Engineering Center, The Johns Hopkins University School of Medicine, 400 N. Broadway/Smith Building Room 5017, Baltimore, Maryland 21231, USA.
Chem Commun (Camb). 2013 Jun 11;49(46):5319-21. doi: 10.1039/c3cc40718g.
Described here is the synthesis and characterization of a novel, bioreducible linear poly(β-amino ester) designed to condense siRNA into nanoparticles and efficiently release it upon entering the cytoplasm. Delivery of siRNA using this polymer achieved near-complete knockdown of a fluorescent marker gene in primary human glioblastoma cells with no cytotoxicity.
本文描述了一种新型的生物可还原线性聚（β-氨基酯）的合成和表征，该聚合物旨在将 siRNA 凝聚成纳米颗粒，并在进入细胞质时有效地释放 siRNA。使用这种聚合物递送 siRNA 可使原代人脑胶质瘤细胞中的荧光标记基因几乎完全沉默，且无细胞毒性。
