Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China.
Front Immunol. 2022 May 2;13:859923. doi: 10.3389/fimmu.2022.859923. eCollection 2022.
Polysaccharides from exhibit a variety of biological activities. Here, we obtained a homogeneous branched β-1,6-glucan (APEP-A-b) from the fruiting bodies of and investigated its effect on immunity and gut microbiota. Our results showed that APEP-A-b significantly increases splenic lymphocyte proliferation, NK cell activity and phagocytic capacity of peritoneal cavity phagocytes. Furthermore, we found that the proportion of CD4 and CD8 T cells in lamina propria are significantly increased upon APEP-A-b treatment. Additionally, APEP-A-b supplementation demonstrated pronounced changes in microbiota reflected in promotion of relative abundances of species in the Lachnospiraceae and Rikenellaceae families. Consistently, APEP-A-b significantly increased the concentration of acetic and butyric acid in cecum contents. Overall, our results suggest that β-1,6-glucan from P. eryngii might enhance immunity by modulating microbiota. These results are important for the processing and product development of P. eryngii derived polysaccharides.
杏鲍菇多糖具有多种生物活性。在这里，我们从杏鲍菇子实体中得到了一种均一的支链β-1,6-葡聚糖（APEP-A-b），并研究了其对免疫和肠道微生物群的影响。我们的结果表明，APEP-A-b 可显著增加脾淋巴细胞增殖、NK 细胞活性和腹腔吞噬细胞的吞噬能力。此外，我们发现 APEP-A-b 处理后，固有层中 CD4 和 CD8 T 细胞的比例明显增加。此外，APEP-A-b 的补充显著改变了微生物群，促进了 Lachnospiraceae 和 Rikenellaceae 科的相对丰度。一致地，APEP-A-b 显著增加了盲肠内容物中乙酸和丁酸的浓度。总的来说，我们的结果表明，杏鲍菇来源的β-1,6-葡聚糖可能通过调节微生物群来增强免疫力。这些结果对杏鲍菇多糖的加工和产品开发具有重要意义。
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