由于实际样本与标准品之间的PCR效率差异导致环境DNA目标定量中的误差估计 - Suppr

Error estimation in environmental DNA targets quantification due to PCR efficiencies differences between real samples and standards.

作者信息

Pérez Leonardo Martín, Fittipaldi Mariana, Adrados Bárbara, Morató Jordi, Codony Francesc

机构信息

Laboratori de Microbiologia Sanitaria i Mediambiental (MSM-Lab) & Aquasost - UNESCO Chair in Sustainability, Universitat Politècnica de Catalunya (UPC), Edifici Gaia, Rambla Sant Nebridi 22, 08222, Terrassa, Barcelona, Spain.

出版信息

Folia Microbiol (Praha). 2013 Nov;58(6):657-62. doi: 10.1007/s12223-013-0255-5. Epub 2013 May 7.

DOI:10.1007/s12223-013-0255-5

PMID:23649952

Abstract

摘要

相似文献

Error estimation in environmental DNA targets quantification due to PCR efficiencies differences between real samples and standards.由于实际样本与标准品之间的PCR效率差异导致环境DNA目标定量中的误差估计

Folia Microbiol (Praha). 2013 Nov;58(6):657-62. doi: 10.1007/s12223-013-0255-5. Epub 2013 May 7.

Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods.通过培养和聚合酶链反应检测冷热水样本中军团菌的效能。I. 方法的标准化

Ann Agric Environ Med. 2012;19(2):289-93.

Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples.临床和环境样本中军团菌检测方案的设计与实施

Diagn Microbiol Infect Dis. 2008 Oct;62(2):125-32. doi: 10.1016/j.diagmicrobio.2008.05.004. Epub 2008 Jul 14.

Usefulness of real-time PCR as a complementary tool to the monitoring of Legionella spp. and Legionella pneumophila by culture in industrial cooling systems.实时 PCR 在工业冷却系统中作为军团菌属和嗜肺军团菌培养监测的补充工具的有用性。

J Appl Microbiol. 2011 Aug;111(2):499-510. doi: 10.1111/j.1365-2672.2011.05063.x. Epub 2011 Jun 23.

Rapid detection of viable Legionella pneumophila in tap water by a qPCR and RT-PCR-based method.基于 qPCR 和 RT-PCR 的方法快速检测自来水中的嗜肺军团菌。

J Appl Microbiol. 2018 Oct;125(4):1216-1225. doi: 10.1111/jam.13932. Epub 2018 Jul 23.

Application of Legionella pneumophila-specific quantitative real-time PCR combined with direct amplification and sequence-based typing in the diagnosis and epidemiological investigation of Legionnaires' disease.应用嗜肺军团菌特异性实时定量 PCR 联合直接扩增和基于序列的分型技术在军团病的诊断和流行病学调查中的应用。

Eur J Clin Microbiol Infect Dis. 2012 Aug;31(8):2017-28. doi: 10.1007/s10096-011-1535-0. Epub 2012 Jan 26.

Enhancement of Legionella pneumophila culture isolation from microenvironments by macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction.通过巨噬细胞感染增强剂（mip）基因特异性巢式聚合酶链反应提高嗜肺军团菌在微环境中的培养分离率。

Microbiol Immunol. 2007;51(8):777-85. doi: 10.1111/j.1348-0421.2007.tb03967.x.

Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR.应用特定实时 PCR 技术检测西班牙水样中的军团菌属和嗜肺军团菌。

Arch Microbiol. 2014 Jan;196(1):63-71. doi: 10.1007/s00203-013-0934-2. Epub 2013 Nov 22.

Multiplex PMA-qPCR Assay with Internal Amplification Control for Simultaneous Detection of Viable Legionella pneumophila, Salmonella typhimurium, and Staphylococcus aureus in Environmental Waters.多重 PMA-qPCR 检测法联合内参扩增，用于同时检测环境水中的活嗜肺军团菌、鼠伤寒沙门氏菌和金黄色葡萄球菌。

Environ Sci Technol. 2015 Dec 15;49(24):14249-56. doi: 10.1021/acs.est.5b03583. Epub 2015 Nov 12.

Design and application of a synthetic DNA standard for real-time PCR analysis of microbial communities in a biogas digester.用于沼气池中微生物群落实时PCR分析的合成DNA标准品的设计与应用

Appl Microbiol Biotechnol. 2015 Aug;99(16):6855-63. doi: 10.1007/s00253-015-6721-z. Epub 2015 Jun 20.

引用本文的文献

Exploring the Impact of Primer-Template Mismatches on PCR Performance of DNA Polymerases Varying in Proofreading Activity.探究校对活性不同的 DNA 聚合酶的引物-模板错配对 PCR 性能的影响。

Genes (Basel). 2024 Feb 7;15(2):215. doi: 10.3390/genes15020215.

Efficiency-corrected PCR quantification for identification of prevalence and load of respiratory disease-causing agents in feedlot cattle.用于鉴定饲养场牛呼吸道疾病病原体流行率和载量的校正效率 PCR 定量。

Aust Vet J. 2022 Nov;100(11):539-549. doi: 10.1111/avj.13200. Epub 2022 Aug 18.

Cadmium Uptake, Gene Activation and Structure of Large-Sized Multi-Domain Metallothioneins in the Terrestrial Door Snail (Gastropoda, Clausiliidae).陆生洞穴蜗牛（腹足纲，巴蜗牛科）中大型多结构域金属硫蛋白的镉摄取、基因激活和结构。

Int J Mol Sci. 2020 Feb 27;21(5):1631. doi: 10.3390/ijms21051631.

Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.去除基于活力 PCR 方法中偏倚的关键因素：综述。

Curr Microbiol. 2020 Apr;77(4):682-687. doi: 10.1007/s00284-019-01829-y. Epub 2019 Dec 6.

Transcript levels of members of the SLC2 and SLC5 families of glucose transport proteins in eel swimbladder tissue: the influence of silvering and the influence of a nematode infection.鳗鱼鳔组织中葡萄糖转运蛋白SLC2和SLC5家族成员的转录水平：镀银的影响和线虫感染的影响。

Fish Physiol Biochem. 2018 Apr;44(2):599-613. doi: 10.1007/s10695-017-0456-y. Epub 2018 Jan 11.

quantGenius: implementation of a decision support system for qPCR-based gene quantification.quantGenius：基于定量聚合酶链反应的基因定量决策支持系统的实现

BMC Bioinformatics. 2017 May 25;18(1):276. doi: 10.1186/s12859-017-1688-7.

Development and Evaluation of qPCR Assay for Quantitation of Kazachstania slooffiae and Total Yeasts Occurring in the Porcine Gut.用于定量猪肠道中斯洛菲卡扎酵母和总酵母的qPCR检测方法的开发与评估

Curr Microbiol. 2015 Sep;71(3):373-81. doi: 10.1007/s00284-015-0862-2. Epub 2015 Jul 2.

本文引用的文献

Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples.用于校正微生物群落样品定量 PCR 效率变化的简单绝对定量方法。

Appl Environ Microbiol. 2012 Jun;78(12):4481-9. doi: 10.1128/AEM.07878-11. Epub 2012 Apr 6.

Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples.定量实时 PCR 分析中标准曲线扩增效率的差异及其对环境样品中基因定量的影响。

J Microbiol Methods. 2010 Sep;82(3):338-41. doi: 10.1016/j.mimet.2010.07.005. Epub 2010 Jul 15.

Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value.基于水解探针的定量 PCR 中观察到的 Cq 值偏倚可以通过估计的 PCR 效率值进行校正。

Methods. 2010 Apr;50(4):313-22. doi: 10.1016/j.ymeth.2010.02.003. Epub 2010 Feb 6.

Statistical aspects of quantitative real-time PCR experiment design.定量实时 PCR 实验设计的统计方面。

Methods. 2010 Apr;50(4):231-6. doi: 10.1016/j.ymeth.2010.01.025. Epub 2010 Jan 28.

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.扩增效率：定量PCR数据分析中基线与偏差的关联

Nucleic Acids Res. 2009 Apr;37(6):e45. doi: 10.1093/nar/gkp045. Epub 2009 Feb 22.

Quantitative real-time PCR is not more sensitive than "conventional" PCR.定量实时聚合酶链反应并不比“传统”聚合酶链反应更灵敏。

J Clin Microbiol. 2008 Jun;46(6):1897-900. doi: 10.1128/JCM.02258-07. Epub 2008 Apr 9.

Development and evaluation of a Taqman duplex real-time PCR quantification method for reliable enumeration of Legionella pneumophila in water samples.用于可靠计数水样中嗜肺军团菌的Taqman双重实时荧光定量PCR方法的开发与评估

J Microbiol Methods. 2007 Jan;68(1):137-44. doi: 10.1016/j.mimet.2006.07.002. Epub 2006 Aug 17.

Development of Bacteroides 16S rRNA gene TaqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water.基于TaqMan的拟杆菌16S rRNA基因实时荧光定量PCR检测方法的开发，用于评估水中的总粪便污染、人类粪便污染和牛粪便污染。

Appl Environ Microbiol. 2006 Jun;72(6):4214-24. doi: 10.1128/AEM.01036-05.

Evaluation of uncertainty in quantitative real-time PCR.定量实时聚合酶链反应中的不确定性评估。

J Microbiol Methods. 2006 Nov;67(2):349-56. doi: 10.1016/j.mimet.2006.04.005. Epub 2006 Jun 2.

Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41.用于检测人腺病毒及鉴定40型和41型血清型的定量实时聚合酶链反应检测法。

Appl Environ Microbiol. 2005 Jun;71(6):3131-6. doi: 10.1128/AEM.71.6.3131-3136.2005.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Error estimation in environmental DNA targets quantification due to PCR efficiencies differences between real samples and standards.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献检索

文件翻译

深度研究

Suppr 超能文献

相似文献

引用本文的文献

本文引用的文献