[电针治疗对阿尔茨海默病大鼠额叶和海马中磷酸化P38丝裂原活化蛋白激酶及白细胞介素-1β表达的影响]

[Effect of electroacupuncture on expression of phosphorylated P 38 MAPK and IL-1beta in frontal lobe and hippocampus in rats with Alzheimer's disease].

作者信息

Fang Jian-Qiao, Zhu Shu-Xiu, Zhang Ying, Wang Fang, Zhu Qing-Yan

机构信息

Zhejiang University of Chinese Medicine, Hangzhou 310005, China.

出版信息

Zhen Ci Yan Jiu. 2013 Feb;38(1):35-9.

PMID:23650798

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) intervention on expression of phosphorylated mitogen activated protein kinase-P 38 (p-P 38 MAPK) protein and Interleukin 1beta (IL-1beta)mRNA in the frontal lobe and hippocampus in Alzheimer's disease (AD) rats so as to explore its mechanisms underlying improvement of AD in clinic.

METHODS

Thirty-two SD rats were equally and randomly divided into normal control (normal), sham-operation (sham), model and EA groups. AD model was established by microinjection of Abeta(1-40) (10 microg/microL, 1 microL) into bilateral Meynert nucleus (AP: 1. 4 mm, L: 3 mm, H: 7 mm) and validated by water maze tests. Rats of the sham group were treated by microinjection of the same dose of normal saline into the bilateral Meynert Nucleus. EA (1 mA, 2 Hz) was applied to bilateral "Baihui" (GV 20), "Taixi" (KI 3) and "Zusanli" (ST 36) for 15 min, once daily for 12 sessions. Expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were detected by Western blot and RT-PCR, respectively.

RESULTS

In comparison with the normal group, the expression levels of p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal lobe tissues were upregulated significantly in the model group (P < 0.01). After 12 sessions of EA intervention, the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA were down-regulated significantly (P < 0.01, P < 0.05) in spite of being still higher than those of the normal group. No significant differences were found between the normal and sham groups in the expression levels of both p-P 38 MAPK protein and IL-1beta mRNA (P > 0.05).

CONCLUSION

EA intervention can reduce the over expression of both p-P 38 MAPK protein and IL-1beta mRNA in the hippocampus and frontal cortex in AD rats, suggesting an improvement of AD after EA intervention by restraining the inflammatory reaction.

摘要

目的

观察电针干预对阿尔茨海默病（AD）大鼠额叶和海马中磷酸化丝裂原活化蛋白激酶 -P38（p-P38 MAPK）蛋白表达及白细胞介素 1β（IL-1β）mRNA 的影响，以探讨其临床改善 AD 的机制。

方法

将 32 只 SD 大鼠平均随机分为正常对照组（正常组）、假手术组（假手术组）、模型组和电针组。通过向双侧 Meynert 核（前囟：1.4 mm，旁开：3 mm，深度：7 mm）微量注射 Aβ（1-40）（10 μg/μL，1 μL）建立 AD 模型，并通过水迷宫试验进行验证。假手术组大鼠通过向双侧 Meynert 核微量注射相同剂量的生理盐水进行处理。电针（1 mA，2 Hz）刺激双侧“百会”（GV 20）、“太溪”（KI 3）和“足三里”（ST 36）15 分钟，每日 1 次，共 12 次。分别采用 Western blot 和 RT-PCR 检测海马和额叶组织中 p-P38 MAPK 蛋白表达水平及 IL-1β mRNA 水平。

结果

与正常组相比，模型组海马和额叶组织中 p-P38 MAPK 蛋白表达水平及 IL-1β mRNA 水平显著上调（P < 0.01）。电针干预 12 次后，p-P38 MAPK 蛋白表达水平及 IL-1β mRNA 水平均显著下调（P < 0.01，P < 0.05），但仍高于正常组。正常组和假手术组在 p-P38 MAPK 蛋白表达水平及 IL-1β mRNA 水平上均无显著差异（P > 0.05）。