Division of Nutritional Sciences, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, USA.
J Agric Food Chem. 2010 Aug 25;58(16):8966-76. doi: 10.1021/jf100975m. Epub 2010 Jul 27.
Interest in berries from South America has increased due to their potential health benefits. The objective of this study was to characterize the anthocyanins and proanthocyanidins of Vaccinium floribundum and Aristotelia chilensis , total phenolics, and antioxidant capacity and to evaluate, in vitro, the ability of their phenolic extracts to reduce adipogenesis and lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory property of these extracts on RAW 264.7 macrophages was also investigated. Antioxidant capacity, measured as oxygen radical scavenging capacity and expressed as Trolox equivalents, was higher in the berries of A. chilensis. Phenolic extracts inhibited lipid accumulation by 4.0-10.8% when adipocytes were treated at maturity and by 5.9-37.9% when treated throughout differentiation. Furthermore, a proanthocyanidin-enriched fraction from V. floribundum significantly increased Pref-1 expression in preadipocytes. Phenolic extracts decreased the production of nitric oxide (3.7-25.5%) and prostaglandin E2 (9.1-89.1%) and the expression of inducible nitric oxide synthase (9.8-61.8%) and cycloxygenase-2 (16.6-62.0%) in lipopolysaccharide-stimulated RAW 264.7 macrophages. V. floribundum and A. chilensis phytochemicals limit adipogenesis and inflammatory pathways in vitro, warranting further in vivo studies.
由于南美洲浆果具有潜在的健康益处，因此人们对其兴趣日益浓厚。本研究的目的是对蔓越莓和智利茶藨子的花色苷和原花青素、总酚和抗氧化能力进行特征分析，并评估其酚类提取物在体外抑制 3T3-L1 脂肪细胞脂肪生成和脂质积累的能力。还研究了这些提取物对 RAW 264.7 巨噬细胞的抗炎特性。抗氧化能力通过氧自由基清除能力来衡量，并用 Trolox 当量表示，智利茶藨子浆果的抗氧化能力更高。当脂肪细胞成熟时，酚类提取物可抑制 4.0-10.8%的脂质积累，当整个分化过程中进行处理时，可抑制 5.9-37.9%的脂质积累。此外，蔓越莓中的原花青素富集部分可显著增加前脂肪细胞中 Pref-1 的表达。酚类提取物可降低脂多糖刺激的 RAW 264.7 巨噬细胞中一氧化氮（3.7-25.5%）和前列腺素 E2（9.1-89.1%）的产生，以及诱导型一氧化氮合酶（9.8-61.8%）和环氧化酶-2（16.6-62.0%）的表达。蔓越莓和智利茶藨子的植物化学物质可限制体外脂肪生成和炎症途径，值得进一步进行体内研究。
