Departamento de Microbiología, Hospital Universitario de Gran Canaria Doctor Negrín, Las Palmas de Gran Canaria, Spain.
Clin Microbiol Infect. 2013 Sep;19(9):E424-7. doi: 10.1111/1469-0691.12248. Epub 2013 May 8.
In a prospective study (2009-2011) in healthcare institutions from the Canary Islands (Spain), 6 out of 298 carbapenem non-susceptible Pseudomonas aeruginosa isolates produced a metallo-β-lactamase: four IMP-15, two VIM-2 (including one IMP-15-positive isolate) and one VIM-1. Multilocus sequence typing identified the single VIM-1-producing isolate as clone ST111 and two IMP-15-producing isolates as ST606, but, strikingly, bacterial re-identification revealed that the other three isolates (producing IMP-15 and/or VIM-2) were actually Pseudomonas putida. Further retrospective analysis revealed a very high prevalence (close to 50%) of carbapenem resistance in this environmental species. Hence, we report the simultaneous emergence in hospitals on the Canary Islands of P. putida and P. aeruginosa strains producing IMP-15, a metallo-β-lactamase not previously detected in Europe, and suggest an underestimated role of P. putida as a nosocomial reservoir of worrying transferable resistance determinants.
在一项针对加那利群岛（西班牙）医疗机构的前瞻性研究（2009-2011 年）中，298 株耐碳青霉烯类铜绿假单胞菌分离株中有 6 株产生了金属β-内酰胺酶：4 株 IMP-15、2 株 VIM-2（包括 1 株 IMP-15 阳性分离株）和 1 株 VIM-1。多位点序列分型将唯一产生 VIM-1 的分离株鉴定为 ST111 克隆，2 株产生 IMP-15 的分离株鉴定为 ST606，但令人惊讶的是，细菌重新鉴定显示其他 3 株（产生 IMP-15 和/或 VIM-2）实际上是铜绿假单胞菌。进一步的回顾性分析显示，这种环境物种的碳青霉烯类耐药性非常高（接近 50%）。因此，我们报告了在加那利群岛的医院中同时出现了产 IMP-15 的铜绿假单胞菌和鲍曼不动杆菌菌株，IMP-15 是一种以前在欧洲未检测到的金属β-内酰胺酶，并提示铜绿假单胞菌作为医院内传播令人担忧的可转移耐药决定因素的作用被低估。
