National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China ; 2University of Chinese Academy of Sciences, Beijing 100049, R.P. China.
Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, DK-2800, Denmark ; Sino-Danish Center for Education and Research, Niels Jensensvej 2, DK-8000, Aarhus C, Denmark.
Biotechnol Biofuels. 2013 May 8;6:74. doi: 10.1186/1754-6834-6-74. eCollection 2013.
Succinic acid is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts. The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid was investigated.
The transgenic E. coli strain could grow in modified M9 medium containing 20 v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.38 g/L succinic acid was produced. With the presence of 4 g/L glucose in the medium, succinic acid concentration increased from 1.4 to 2.4 g/L by addition of 20 v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.3 g/L succinic acid was produced. The obtained succinic acid concentration increased to 11.5 g/L when 12.5 v/v% AP-bio-oil was added. However, it decreased to 8 g/L when 50 v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli.
The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid production.
琥珀酸是一种关键的平台化学品，可以通过生物技术过程而不是石化过程生产。生物量衍生的生物油已被广泛研究作为柴油和汽油燃料的替代品。生物油可以分为有机相和水相两部分。有机相生物油可以很容易地升级为运输燃料。水相生物油（AP-生物油）价值较低。目前尚无关于其通过生物方法使用或升级的报道。本文研究了利用 AP-生物油生产琥珀酸。
转 E. coli 菌株能够在含有 20 v/v%AP-生物油的改良 M9 培养基中生长，OD 从 0.25 增加到 1.09。并产生 0.38 g/L 的琥珀酸。在培养基中存在 4 g/L 葡萄糖的情况下，通过添加 20 v/v%AP-生物油，琥珀酸浓度从 1.4 增加到 2.4 g/L。当使用玉米秸秆的酶解产物作为碳源时，可生产 10.3 g/L 的琥珀酸。当添加 12.5 v/v%AP-生物油时，得到的琥珀酸浓度增加到 11.5 g/L。然而，当添加 50 v/v%AP-生物油时，浓度降低至 8 g/L。GC-MS 分析表明，AP-生物油中的一些低分子碳化合物被 E. coli 利用。
结果表明，AP-生物油可被 E. coli 用于细胞生长和琥珀酸生产。
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