Lee P C, Lee S Y, Hong S H, Chang H N
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea.
Appl Microbiol Biotechnol. 2002 Apr;58(5):663-8. doi: 10.1007/s00253-002-0935-6. Epub 2002 Feb 8.
A novel succinic acid-producing bacterium was isolated from bovine rumen. The bacterium is a non-motile, non-spore-forming, mesophilic and capnophilic gram-negative rod or coccobacillus. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the recently reclassified genus Mannheimia as a novel species, and has been named Mannheimia succiniciproducens MBEL55E. Under 100% CO(2) conditions, it grows well in the pH range of 6.0-7.5 and produces succinic acid, acetic acid and formic acid at a constant ratio of 2:1:1. When M. succiniciproducensMBEL55E was cultured anaerobically in medium containing 20 g l(-1) glucose as carbon source, 13.5 g l(-1) of succinic acid was produced.
从牛瘤胃中分离出一种新型产琥珀酸细菌。该细菌为无运动性、不形成芽孢、嗜温且嗜二氧化碳的革兰氏阴性杆菌或球杆菌。基于16S rRNA序列的系统发育分析和生理分析表明，该菌株属于最近重新分类的曼氏杆菌属的一个新物种，已被命名为琥珀酸曼氏杆菌MBEL55E。在100%二氧化碳条件下，它在pH值6.0 - 7.5范围内生长良好，并以2:1:1的恒定比例产生琥珀酸、乙酸和甲酸。当琥珀酸曼氏杆菌MBEL55E在含有20 g l(-1)葡萄糖作为碳源的培养基中厌氧培养时，可产生13.5 g l(-1)的琥珀酸。
