杏仁皮对 cagA 阳性和阴性的幽门螺杆菌临床分离株的抗菌活性。
Antibacterial activities of almond skins on cagA-positive and-negative clinical isolates of Helicobacter pylori.
出版信息
BMC Microbiol. 2013 May 9;13:103. doi: 10.1186/1471-2180-13-103.
BACKGROUND
Helicobacter pylori is known to be a gastric pathogen of humans. Eradication regimens for H. pylori infection have some side effects, compliance problems, relapses, and antibiotic resistance. Therefore, the need for alternative therapies for H. pylori infections is of special interest. We have previously shown that polyphenols from almond skins are active against a range of food-borne pathogens. The aim of this study was to evaluate the antibacterial effects of natural almond skins before and after simulated human digestion and the pure flavonoid compounds epicatechin, naringenin and protocatechuic acid against H. pylori.
RESULTS
H. pylori strains were isolated from gastric biopsy samples following standard microbiology procedures. Also, cagA and vacA genes were identified using PCR. Susceptibility studies on 34 strains of H. pylori, including two reference strains (ATCC 43504, ATCC 49503), were performed by the standard agar dilution method.
CONCLUSIONS
Polyphenols from almond skins were effective in vitro against H. pylori, irrespective of genotype status and could therefore be used in combination with antibiotics as a novel strategy for antibiotic resistance.
背景
已知幽门螺杆菌是人类的胃病原体。根除幽门螺杆菌感染的方案有一些副作用、顺应性问题、复发和抗生素耐药性。因此，人们对替代幽门螺杆菌感染的疗法特别感兴趣。我们之前已经表明，杏仁皮中的多酚对一系列食源性病原体具有活性。本研究的目的是评估天然杏仁皮在模拟人体消化前后以及纯类黄酮化合物表儿茶素、柚皮苷和原儿茶酸对幽门螺杆菌的抗菌作用。
结果
按照标准微生物学程序从胃活检样本中分离出幽门螺杆菌菌株。此外，还通过 PCR 鉴定了 cagA 和 vacA 基因。采用标准琼脂稀释法对包括两个参考菌株（ATCC 43504、ATCC 49503）在内的 34 株幽门螺杆菌菌株进行了药敏研究。
结论
杏仁皮中的多酚对幽门螺杆菌具有体外有效性，无论基因型状态如何，因此可以与抗生素联合使用，作为一种新的抗生素耐药策略。
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