Department of Immunology, Genetics and Immunology, Uppsala University, Uppsala, Sweden.
Virology. 2013 Aug 1;442(2):148-55. doi: 10.1016/j.virol.2013.04.006. Epub 2013 May 6.
Using deep RNA sequencing, we have studied the expression of adenovirus-encoded small RNAs at different times after infection. Nineteen small RNAs which comprised more than 1% of the total pool of small RNAs at least one time point were identified. These small RNAs were between 25 and 35 nucleotides long and mapped in the region of the VA RNAI and RNAII genes. However, the overlap was incomplete and some contained a few extra nucleotides at the 3' end. This finding together with the observation that some of the small RNAs were detected before VA RNA expression had started might indicate that they are derived from other precursors than VA RNAI and II. Interestingly, the small RNAs displayed different expression profiles during the course of the infection suggesting that they have different functions. An effort was made to identify their mRNA targets by using computer prediction and deep cDNA sequencing. The most significant targets for the earliest small RNAs were genes involved in signaling pathways.
利用深度 RNA 测序，我们研究了感染后不同时间腺病毒编码的小 RNA 的表达情况。鉴定出了 19 种小 RNA，它们至少在一个时间点占小 RNA 总池的 1%以上。这些小 RNA 长 25 到 35 个核苷酸，在 VA RNAI 和 RNAII 基因区域定位。然而，重叠并不完整，一些在 3'端有几个额外的核苷酸。这一发现，再加上一些小 RNA 在 VA RNA 表达开始之前就被检测到的观察结果，可能表明它们来自 VA RNAI 和 II 以外的其他前体。有趣的是，小 RNA 在感染过程中表现出不同的表达谱，这表明它们具有不同的功能。我们通过计算机预测和深度 cDNA 测序努力识别它们的 mRNA 靶标。最早的小 RNA 的最显著靶标是参与信号通路的基因。
